Lisa H. Orfe

Microcin PDI regulation and proteolytic cleavage are unique among known microcins

Zhe Zhao, Lisa H. Orfe, Jinxin Liu, Shao-Yeh Lu et autres

Microcin PDI inhibits a diversity of pathogenic Escherichia coli through the action of an effector protein, McpM. In this study we demonstrated that expression of the inhibitory phenotype is induced under low osmolarity conditions and expression is primarily controlled by the EnvZ/OmpR …

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ExsE Is a Negative Regulator for T3SS Gene Expression in Vibrio alginolyticus

Jinxin Liu, Shao-Yeh Lu, Lisa H. Orfe, Chunhua Ren et autres

Type III secretion systems (T3SSs) contribute to microbial pathogenesis of Vibrio species, but the regulatory mechanisms are complex. We determined if the classic ExsACDE protein-protein regulatory model from Pseudomonas aeruginosa applies to Vibrio alginolyticus. Deletion mutants in V. alginolyticus demonstrated that, as …

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Soil‐borne reservoirs of antibiotic‐resistant bacteria are established following therapeutic treatment of dairy calves

Jinxin Liu, Zhe Zhao, Lisa H. Orfe, Douglas Ruben Call

We determined if antibiotics residues that are excreted from treated animals can contribute to persistence of resistant bacteria in agricultural environments. Administration of ceftiofur, a third-generation cephalosporin, resulted in a ∼ 3 log increase in ceftiofur-resistant Escherichia coli found in the faeces …

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Potential mechanisms of attenuation for rifampicin-passaged strains of Flavobacterium psychrophilum

Karol Gliniewicz, Mark R. Wildung, Lisa H. Orfe, Gregory D. Wiens et autres

BACKGROUND: Flavobacterium psychrophilum is the etiologic agent of bacterial coldwater disease in salmonids. Earlier research showed that a rifampicin-passaged strain of F. psychrophilum (CSF 259-93B.17) caused no disease in rainbow trout (Oncorhynchus mykiss, Walbaum) while inducing a protective immune response against challenge …

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Genome-Wide Screening Identifies Six Genes That Are Associated with Susceptibility to Escherichia coli Microcin PDI

Zhe Zhao, Lauren J. Eberhart, Lisa H. Orfe, Shao-Yeh Lu et autres

The microcin PDI inhibits a diverse group of pathogenic Escherichia coli strains. Coculture of a single-gene knockout library (BW25113; n=3,985 mutants) against a microcin PDI-producing strain (E. coli 25) identified six mutants that were not susceptible (ΔatpA, ΔatpF, ΔdsbA, ΔdsbB, ΔompF, and …

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The mechanism of neutral red-mediated microbial electrosynthesis in Escherichia coli: menaquinone reduction

Timothy D. Harrington, Vi N. Tran, Abdelrhman H. Mohamed, Ryan Scott Renslow et autres

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Additional file 1: Figure S1. of Potential mechanisms of attenuation for rifampicin-passaged strains of Flavobacterium psychrophilum

Karol Gliniewicz, Mark R. Wildung, Lisa H. Orfe, Gregory D. Wiens et autres

SDS-PAGE analysis of carbohydrate extractions from different F. psychrophilum CSF 259-93 and THC 02-90 strains. Table S1: Single nucleotide polymorphisms (SNPs) from F. psychrophilum THC 02-90 strain passaged without rifampicin (TN). Table S2: Single nucleotide polymorphisms (SNPs) from F. psychrophilum THC 02-90 …

Additional file 1: Figure S1. of Potential mechanisms of attenuation for rifampicin-passaged strains of Flavobacterium psychrophilum

Karol Gliniewicz, Mark Wildung, Lisa H. Orfe, Gregory D. Wiens et autres

SDS-PAGE analysis of carbohydrate extractions from different F. psychrophilum CSF 259-93 and THC 02-90 strains. Table S1: Single nucleotide polymorphisms (SNPs) from F. psychrophilum THC 02-90 strain passaged without rifampicin (TN). Table S2: Single nucleotide polymorphisms (SNPs) from F. psychrophilum THC 02-90 …

Additional file 1: Figure S1. of Potential mechanisms of attenuation for rifampicin-passaged strains of Flavobacterium psychrophilum

Karol Gliniewicz, Mark Wildung, Lisa H. Orfe, Gregory D. Wiens et autres

SDS-PAGE analysis of carbohydrate extractions from different F. psychrophilum CSF 259-93 and THC 02-90 strains. Table S1: Single nucleotide polymorphisms (SNPs) from F. psychrophilum THC 02-90 strain passaged without rifampicin (TN). Table S2: Single nucleotide polymorphisms (SNPs) from F. psychrophilum THC 02-90 …

Entericidin Is Required for a Probiotic Treatment (Enterobacter sp. Strain C6-6) To Protect Trout from Cold-Water Disease Challenge

Carla B. Schubiger, Lisa H. Orfe, Ponnerassery S. Sudheesh, Kenneth D. Cain et autres

Flavobacterium psychrophilum causes bacterial cold-water disease in multiple fish species, including salmonids. An autochthonous Enterobacter strain (C6-6) inhibits the in vitro growth of F. psychrophilum, and when ingested as a putative probiotic, it provides protection against injection challenge with F. psychrophilum in …

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Ciprofloxacin Residues in Municipal Biosolid Compost Do Not Selectively Enrich Populations of Resistant Bacteria

Caitlin Price Youngquist, Jinxin Liu, Lisa H. Orfe, Stephen Scott Jones et autres

Biosolids and livestock manure are valuable high-carbon soil amendments, but they commonly contain antibiotic residues that might persist after land application. While composting reduces the concentration of extractable antibiotics in these materials, if the starting concentration is sufficiently high then remaining residues …

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Imported food can serve as a fomite for antibiotic resistance genes

Douglas Ruben Call, Margaret A. Davis, Deven Tokuno, Lisa H. Orfe

We selected uncooked farmed shrimp from Thailand and Indonesia and fresh wild shrimp from Mexico and the United States for these studies. We extracted bulk DNA from these products and introduced the DNA to a lab strain of E. coli by electroporation …