Accès ouvert
2026
preprint
OpenAlex
Cheryl Kim, Denise Hinz, Aaron J. Middlebrook, Aaron J. Tyznik et autres
Abstract Circulating cell-cell complexes (doublets) provide critical insights into in vivo immune interactions. Here, we leverage high-dimensional imaging spectral cytometry to systematically map the landscape of immune doublets in human peripheral blood. By integrating morphometric imaging features with a 22-color spectral panel, …
us, Afrique du Sud
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Ningxin Kang, Cheryl Kim, Bjoern Peters, Julie G. Burel
Although routinely detected in human blood and tissue samples, immune cell doublets are typically discarded during data acquisition or analysis, often dismissed as technical artifacts arising from ex vivo cell handling. However, emerging evidence suggests that these immune cell doublets reflect biologically …
us
(code pays fourni par la source)
2026
article
OpenAlex
Ningxin Kang, Cheryl Kim, Thomas Scriba, Bjoern Peters et autres
Abstract Introduction Communication between immune cells through direct contact is a critical feature of immune responses. Our previous work has highlighted that a significant fraction of immune cell doublets detected in non-imaging flow cytometry are not technical artifacts, and instead hold biological …
us, Afrique du Sud
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Paul K. Wallace, Evan R. Jellison, Sherry Thornton, Kathleen Kluepfel et autres
joint effort begun in 2018 and continued in 2019 by Kamila Czechowska proved to be a valuable reference [1,2].Building on that spirit, we present summaries from CYTO 2025, held in Denver, Colorado, from May 31 to June 4.This manuscript serves as a …
us, uy, ca, de, au, nl
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Paul K. Wallace, Evan R. Jellison, Sherry Thornton, Kathleen Kluepfel et autres
joint effort begun in 2018 and continued in 2019 by Kamila Czechowska proved to be a valuable reference [1,2].Building on that spirit, we present summaries from CYTO 2025, held in Denver, Colorado, from May 31 to June 4.This manuscript serves as a …
us, gb, de, uy, be, ca, in, dk, es, nl, au, nz, ch, pt
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Julie G. Burel, Ningxin Kang, Cheryl Kim, Denise Hinz et autres
Abstract Description We have previously demonstrated that 1) many immune cell doublets fail to be categorized as such by conventional flow cytometry analyzers and sorters, 2) imaging parameters are the best features to distinguish doublets from singlets, 3) a significant fraction of …
us
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Yuko Tada, Sujit Silas Armstrong Suthahar, Payel Roy, Vasantika Suryawanshi et autres
Interferon-γ (IFNG) is thought to play a central role in the pathogenesis of cardiac allograft vasculopathy (CAV) in heart transplant (HTx) patients. However, peripheral lymphocytes participating in the IFNG-axis remain largely unknown in human CAV. Using peripheral blood mononuclear cells (PBMC) from …
us, de
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Ningxin Kang, Ashu Chawla, Hannah Hillman, Rashmi Tippalagama et autres
Communication between immune cells through direct contact is a critical feature of immune responses. Here, we developed an innovative high-throughput workflow to study the transcriptome and adaptive immune receptor repertoire of single cells forming complexes without needing bioinformatic deconvolution. We found that …
us, Afrique du Sud, lk, dk
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Gertrude Ecklu-Mensah, Rachel Miller, Maria Gjerstad Maseng, Vienna Hawes et autres
Fermented foods are becoming more popular due to their purported links to metabolic health and the gut microbiome. However, direct clinical evidence for the health claims is lacking. Here, we describe an eight-week clinical trial that explored the effects of a four-week …
us, no
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Yuko Tada, Sujit Silas Armstrong Suthahar, Payel Roy, Vasantika Suryawanshi et autres
Abstract Background T cell mediated immunity is reported to play a pathogenic role in cardiac allograft vasculopathy (CAV) in heart transplant (HTx) patients. However, peripheral blood CD8 + T cells have not been previously characterized in CAV. This study aimed to identify …
us
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Ningxin Kang, Ashu Chawla, Hannah Hillman, Rashmi Tippalagama et autres
Communication between immune cells through direct contact is a critical feature of immune responses. Here, we developed a novel high-throughput method to study the transcriptome and adaptive immune receptor repertoire of single cells forming complexes without needing bioinformatic deconvolution. We found that …
us, Afrique du Sud, lk
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Gertrude Ecklu-Mensah, Rachel Miller, Maria Gjerstad Maseng, Vienna Hawes et autres
Abstract Fermented foods are surging in popularity globally due to their links to metabolic health and the gut microbiome. However, direct clinical evidence for the health claims is lacking. Here, we describe an eight-week clinical trial that explored the effects of a …
us, no
(code pays fourni par la source)