Whole-Genome Sequencing and Intestinal Metagenome Sequencing Revealed the Carriage and Transmission of Salmonella enterica in Xinjiang, China
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Le résumé fourni par la source
Background: Salmonella enterica, a major foodborne pathogen, poses a severe global public health threat. However, data regarding its carriage characteristics, transmission patterns, and association with intestinal microbiota in healthy populations of Xinjiang, China, remain insufficient, limiting the formulation of targeted salmonellosis prevention and control strategies. Methods: In this study, 31 Salmonella enterica strains isolated from more than 2000 healthy individuals in Urumqi were subjected to whole-genome sequencing to analyze serovars, antimicrobial resistance (AMR) genes, and virulence genes. Meanwhile, metagenomic sequencing was performed on 50 fecal samples (culture negative) from 2000 healthy individuals to investigate intestinal microbiota structure, Salmonella enterica prevalence, and related microbial taxa. Results: The results showed that 60% of samples (30/50) were positive by the read-based criterion (≥1000 Salmonella-specific reads), while the assembly-verified criterion (≥1000 reads and contigs > 1 kb) confirmed Salmonella-specific sequences in 12% (6/50). Among the 31 culture-confirmed isolates, Salmonella Typhimurium and Salmonella Paratyphi B were the dominant serovars, together accounting for 60%. All isolates harbored core virulence genes for Type III secretion system and adhesion factors, with low AMR gene carriage and no multidrug-resistant strains. Phylogenetic analysis showed that Urumqi-derived isolates were distributed across multiple genomic clusters, suggesting active inter-regional circulation of S. enterica within the available dataset. Salmonella enterica carriage did not affect gut microbial α-diversity but altered community composition, with Escherichia coli, Shigella flexneri, and Klebsiella pneumoniae as key associated taxa. Conclusions: This study found that Urumqi-derived isolates are widely distributed across genomic clusters in Xinjiang, with a unique local transmission chain identified, though definitive source attribution requires further geographically balanced sampling. Salmonella enterica carriage exhibited ecological niche synergy with intestinal Enterobacteriaceae, but did not significantly affect gut microbial diversity.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Whole-Genome Sequencing and Intestinal Metagenome Sequencing Revealed the Carriage and Transmission of Salmonella enterica in Xinjiang, China
- Date Crossref
- 03/09/2026
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Xinjiang Uygur Autonomous Region Disease Prevention and Control Center pays non établi dans la noticeOrganisme public
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Chinese Center For Disease Control and Prevention pays non établi dans la noticeOrganisme public
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National Institute for Communicable Disease Control and Prevention pays non établi dans la noticeOrganisme public
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Xinjiang Medical University pays non établi dans la noticeUniversité ou école supérieure
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Institute of Pathogenic Biological Detection pays non établi dans la noticeStructure de recherche
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Institute of Infectious Disease Prevention and Control Administration Office pays non établi dans la noticeStructure de recherche
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Research and Technology Transfer Department pays non établi dans la noticeInstitution
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Public Health College pays non établi dans la noticeUniversité ou école supérieure
Xinjiang Uygur Autonomous Region Disease Prevention and Control Center, Chinese Center For Disease Control and Prevention et National Institute for Communicable Disease Control and Prevention, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.