RAB1 mediates OPTN-dependent mitophagy via ATG9A recruitment

Le résumé fourni par la source

: AO: antimycin A and oligomycin; ASH: assembly helper; ATG: autophagy related; CALCOCO2: calcium-binding and coiled-coil domain-containing protein 2; CC: coiled coil; ER: endoplasmic reticulum; Fluoppi: fluorescence-based protein-protein interaction; GABARAP: GABA type A receptor-associated protein; GAP: GTPase-activating protein; GEF: guanine nucleotide exchange factor; HAG: humanized azami-green; IB: immunoblotting; ipTM: interface predicted template modeling score; IQR: interquartile range; KO: knockout; LIR: LC3-interacting region; LRRK2: leucine rich repeat kinase 2; LZ: leucine zipper; MAP1LC3: microtubule associated protein 1 light chain 3; N.D.:not detectable; OPTN: optineurin; PBS: phosphate-buffered saline; PINK1: PTEN-induced kinase 1; PLDDT: predicted local distance difference test; PRKN: Parkin RBR E3 ubiquitin protein ligase; PtdIns3K: phosphatidylinositol 3-kinase; PtdIns3P: phosphatidylinositol 3-phosphate; pTM: predicted template modeling score; PVDF: polyvinylidene difluoride; RAB: RAB, member RAS oncogene family; RB1CC1: RB1 inducible coiled-coil protein 1; SDS: sodium dodecyl sulfate; siRNA: small interfering RNA; TAX1BP1: Tax1 binding protein 1; TBC1D17: TBC1 domain family member 17; TBK1: TANK-binding kinase 1; TKO: triple knockout; TOMM20: translocase of outer mitochondrial membrane 20; Ub: ubiquitin; UBAN: ubiquitin binding in ABIN proteins and NEMO; WIPI: WD-repeat domain phosphoinositide-interacting; WT: wild-type.

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