Isoquercetin targets histone methyltransferase G9a to promote BDNF expression and improve cerebral ischemia-reperfusion injury
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Le résumé fourni par la source
Reperfusion therapies following stroke can contribute to further brain injury.Inhibition of G9a has been demonstrated to increase BDNF expression, thereby exerting neuroprotective effects. Isoquercetin (ISO) has shown neuroprotective potential, but its molecular mechanisms are not fully known. This study aimed to investigate the role and mechanism of ISO in improving cerebral ischemia-reperfusion injury, in relation to G9a/H3K9me2/BDNF pathway. A rat middle cerebral artery occlusion (MCAO) model and an oxygen-glucose deprivation/reoxygenation (OGD/R) neuronal model were used. Neurological scores, infarct volume, brain water content, neuronal morphology, apoptosis and cell viability were evaluated. Moreover, a G9a overexpression plasmid was constructed to verify mechanism specificity. G9a and H3K9me2 expression levels were assessed by RT-PCR and western blotting. BDNF levels were measured by ELISA, oxidative stress by ROS assays and H3K9me2 enrichment at the BDNF promoter by chromatin immunoprecipitation. Cerebral I/R injury significantly increased G9a and H3K9me2 expression, leading to reduced BDNF levels and enhanced neuronal apoptosis. ISO treatment decreased G9a/H3K9me2 expression, increased BDNF levels, improved neurological function and reduced neuronal damage both in vivo and in vitro. G9a overexpression reversed these protective effects. Isoquercetin alleviates cerebral I/R injury by inhibiting G9a-mediated histone methylation and promoting BDNF expression, highlighting a novel epigenetic mechanism for stroke therapy.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Isoquercetin targets histone methyltransferase G9a to promote BDNF expression and improve cerebral ischemia-reperfusion injury
- Date Crossref
- 01/10/2026
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Hainan Medical University Department of Emergency & Key Laboratory of Emergency and Trauma of Ministry of Education pays non établi dans la noticeUniversité ou école supérieure
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Second Military Medical University pays non établi dans la noticeUniversité ou école supérieure
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Shanghai University of Medicine and Health Sciences pays non établi dans la noticeUniversité ou école supérieure
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Shanghai Pudong New Area Gongli Hospital pays non établi dans la noticeÉtablissement de santé
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Shanghai University of Medicine & Health Science Gongli Hospital of Shanghai Pudong New Area pays non établi dans la noticeUniversité ou école supérieure
Department of Emergency & Key Laboratory of Emergency and Trauma of Ministry of Education — Hainan Medical University, Second Military Medical University et Shanghai University of Medicine and Health Sciences, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.