Excessive estrogenic environment during preimplantation disrupts embryo transport and development and differentially alters the transcriptomes of mouse oviduct and uterus
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Le résumé fourni par la source
Objective: Exposure to estrogenic endocrine-disrupting chemicals (EEDCs) has been associated with oviductal embryo retention, also called the tube-locking effect, which heightens the risk of infertility and ectopic pregnancy. Here we used preimplantation 17β-estradiol (E 2 ) exposure as a model to investigate the mechanisms underlying the EEDC-induced tube-locking effect. Methods: Pregnant mice were subcutaneously treated with 0, 0.1, 1, or 10 µg/kg E 2 daily from day 0.5 post-coitum (D0.5) to D2.5. Their oviducts and uteri were analyzed on D3.5 using embryo flushing, histology, microarray, and real-time PCR. Results: Preimplantation E 2 treatment caused dose-dependent oviductal embryo retention and developmental delay. Transcriptomic profiling identified 33 upregulated and 24 downregulated oviductal differentially expressed genes (DEGs)/probesets and 1115 upregulated and 1004 downregulated uterine DEGs/probesets following treatment with 10 µg/kg/day E 2 (fold change >1.5 and q-value <5%). Gene Ontology (GO) analysis revealed E 2 -induced upregulation of the immune response in the oviduct and uterus, whereas ion transport was downregulated in the oviduct but upregulated in the uterus. E 2 -downregulated oviductal DEGs were also found to be involved in metabolism, nutrient transport, and muscle-related functions. Selected oviductal and uterine co-DEGs were confirmed by real-time PCR. Their dose-response patterns following preimplantation E 2 treatment were opposite to their temporal expression patterns in the uterus during preimplantation, but such opposing patterns were less consistent in the oviduct. Conclusions: The oviduct shows limited transcriptional responsiveness but marked functional susceptibility to an excessively estrogenic environment during early pregnancy. E 2 -responsive oviductal DEGs implicate dysregulation of immune homeostasis, fluid and nutrient transport, and muscle-related functions as potential contributors to the tube-locking effect associated with excessive EEDC exposure and the related risks of infertility and ectopic pregnancy.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Excessive estrogenic environment during preimplantation disrupts embryo transport and development and differentially alters the transcriptomes of mouse oviduct and uterus
- Date Crossref
- 18/08/2026
- Éditeur
- Ovid Technologies (Wolters Kluwer Health)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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The State University of New Jersey Rutgers pays non établi dans la noticeUniversité ou école supérieure
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University of Georgia Interdisciplinary Toxicology Program pays non établi dans la noticeUniversité ou école supérieure
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Environmental and Occupational Health Sciences Institute pays non établi dans la noticeUniversité ou école supérieure
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Hubei University of Medicine Reproductive Medical Center pays non établi dans la noticeUniversité ou école supérieure
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University of Nevada Department of Physiology and Cell Biology pays non établi dans la noticeUniversité ou école supérieure
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Ernest Mario School of Pharmacy Department of Pharmacology and Toxicology pays non établi dans la noticeUniversité ou école supérieure
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College of Veterinary Medicine Department of Physiology and Pharmacology pays non établi dans la noticeUniversité ou école supérieure
Rutgers — The State University of New Jersey, Interdisciplinary Toxicology Program — University of Georgia et Environmental and Occupational Health Sciences Institute, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.