Simultaneous mesoscopic imaging of neural activity and hemodynamics reveals anesthetic modulation of neurovascular responses

Le résumé fourni par la source

Anesthesia substantially alters neural activity and vascular tone, yet its state-dependent modulation of cortical neurovascular coupling (NVC) remains incompletely characterized. Here, using an established multimodal optical imaging platform that synchronizes wide-field fluorescence microscopy, photoacoustic microscopy, and laser speckle contrast imaging, we investigated anesthesia-dependent modulation of cortical neurovascular dynamics in mice. We characterized neurovascular responses under isoflurane (ISO), dexmedetomidine (Dex), and low-dose ISO-Dex, and used focal 4-aminopyridine (4-AP) injections to examine seizure-associated responses across awake, ISO, and ISO-Dex conditions. Isoflurane suppressed neural activity, elevated vascular baselines, and induced a neurovascular washout that markedly attenuated seizure-related hemodynamic responses. Dexmedetomidine was associated with relatively preserved neural activity but induced vasoconstriction. In contrast, ISO-Dex reduced ISO-associated vasodilation and retained measurable seizure-associated neurovascular dynamics within this 4-AP seizure paradigm. These results demonstrate that anesthetic state is an active determinant of neurovascular signal fidelity and should be carefully considered when interpreting functional neurovascular imaging data.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.