Laser-Generated Ultrashort Pulsed Electron Beams Induce p53-Related Alteration of DNA Repair and Cell Death Pathways in Non-Small Cell Lung Cancer Cells
Rattachement africain : ru, am. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Laser-driven accelerated particle beams have significantly advanced cancer treatment by facilitating the delivery of exceptionally high dose rates of radiation to solid tumors within femto- to picosecond timescales. This investigation compared the radiobiological effectiveness of ultrashort pulsed electron beams, generated by the Advanced Research Electron Accelerator Laboratory (AREAL) accelerator, with conventional X-rays on two non-small cell lung cancer (NSCLC) cell lines: A549 (wild-type p53) and H1299 (p53-deficient). NSCLC cells were irradiated using either the AREAL accelerator (with a peak dose rate of 1.6 × 1010 Gy/s, a pulse duration of 4.5 × 10−13 s, and a repetition rate of 20 Hz) or an X-ray unit at an absorbed dose rate of 0.85 Gy/min. Clonogenic survival analysis, γH2AX foci enumeration, and genome-wide transcriptome analysis were conducted. Clonogenic survival curves showed increased radiosensitivity of A549 cells following AREAL exposure compared to X-rays (RBE = 1.2), whereas H1299 radiosensitivity remained unchanged. In both cell lines, AREAL exposure resulted in a greater dose-dependent accumulation of residual γH2AX foci 24 h after irradiation than conventional X-rays, suggesting more persistent DNA damage signaling. Transcriptomic analyses revealed broader gene expression changes after AREAL irradiation and suggested distinct p53-related responses. Pathway-level analysis demonstrated that A549 cells exhibited reduced DNA repair activity, accompanied by dysregulation of cell cycle progression and apoptosis, whereas H1299 cells displayed transcriptomic signatures consistent with enhanced homologous recombination activity. Overall, these findings indicate that ultrashort pulsed electron beams induce p53-related responses distinct from those of conventional X-rays and warrant further investigation of this technology as a potential radiotherapy modality.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Laser-Generated Ultrashort Pulsed Electron Beams Induce p53-Related Alteration of DNA Repair and Cell Death Pathways in Non-Small Cell Lung Cancer Cells
- Date Crossref
- 13/08/2026
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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State Scientific Center of the Russian Federation - Federal Medical Biophysical Center named after A.I. Burnazyan pays non établi dans la noticeStructure de recherche
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Federal Medical-Biological Agency pays non établi dans la noticeOrganisme public
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Semenov Institute of Chemical Physics pays non établi dans la noticeStructure de recherche
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A. Alikhanyan National Laboratory pays non établi dans la noticeStructure de recherche
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Yerevan State University Research Institute of Biology pays non établi dans la noticeUniversité ou école supérieure
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Center for the Advancement of Natural Discoveries using Light Emission pays non établi dans la noticeStructure de recherche
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Institute of Chemical Physics NAS RA pays non établi dans la noticeStructure de recherche
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Institute of Biochemical Physics NM Emanuel pays non établi dans la noticeStructure de recherche
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Moscow Center for Advanced Studies pays non établi dans la noticeInstitution
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N.N. Semenov Research Center of Chemical Physics pays non établi dans la noticeStructure de recherche
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CANDLE Synchrotron Research Institute pays non établi dans la noticeStructure de recherche
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Institute of Molecular Biology NAS RA pays non établi dans la noticeStructure de recherche
State Scientific Center of the Russian Federation - Federal Medical Biophysical Center named after A.I. Burnazyan, Federal Medical-Biological Agency et Semenov Institute of Chemical Physics, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.