Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1
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Le résumé fourni par la source
The XPG endonuclease is crucial for nucleotide excision repair (NER) and other genome maintenance pathways. Precise regulation of XPG recruitment and activity during DNA repair is essential to avoid erroneous DNA incisions and genomic instability. In this study, we employed live-cell imaging to investigate how XPG is regulated during NER, focusing on its dynamic interactions with key factors involved in the pre- and post-incision steps. We found that TFIIH and XPA facilitate recruitment and association of XPG with DNA damage and that XPG localizes separately from TFIIH to UV-induced lesions. Furthermore, our results show that XPG's dissociation from DNA damage is triggered by its own incision activity as well as by that of XPF. Additionally, the exonuclease EXO1 promotes XPG dissociation, likely by processing incised DNA, even in the absence of XPG-mediated incision. Our findings help to better understand the regulatory mechanisms that control XPG activity during NER and provide important insights into the complex dynamics of the repair process.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1
- Date Crossref
- 30/06/2026
- Éditeur
- Rockefeller University Press
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Erasmus MC Cancer Institute pays non établi dans la noticeÉtablissement de santé
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Erasmus University Rotterdam pays non établi dans la noticeUniversité ou école supérieure
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Erasmus University Medical Center 1 Department of Molecular Genetics Erasmus MC Cancer Institute pays non établi dans la noticeUniversité ou école supérieure
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Erasmus Medical Center 2 Optical Imaging Centre pays non établi dans la noticeÉtablissement de santé
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Erasmus University Medical Center 3 Department of Pathology Erasmus MC Cancer Institute pays non établi dans la noticeUniversité ou école supérieure
Erasmus MC Cancer Institute, Erasmus University Rotterdam et Erasmus MC Cancer Institute — Erasmus University Medical Center 1 Department of Molecular Genetics, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.