Additional file 2 of CRISPR and compound screens in a novel ex vivo tissue model identify DDR1 and ETA as regulators of cancer cell invasion
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Le résumé fourni par la source
Additional file 1: SF1. Control experiment showing that only UMUC3 cells but not RT4 cells transmigrate through the mouse bladder in the EXTIM A. Representative images illustrating the different transmigratory capacities of three different cell groups, RT4, UMUC3, and RT4:UMUC3-eGFP, in the EXTIM. To evaluate whether any RT4 cells could transmigrate through the mouse blade when cocultured with UMUC3 cells, RT4 cells were seeded with eGFP-expressing UMUC3 cells (UMUC3-eGFP) at a 1000-to-1 ratio in mouse bladders. Only UMUC3 parental cells and UMUC3-eGFP cells transmigrated through the mouse bladder and proliferated to form colonies in the culture plates. These cells were fixed and stained in the wells for visualization. n = 3 independent experiments were performed. Note that, for each cell group, only one of the repeat experiments is shown (depicted here are example plates of the repeat experiments with the plates showing no colonies until day 66 for the RT4 cell group and example plates of the repeat experiments with the plates showing colonies on day 60 for the UMUC3 cell group and RT4 mixed with the UMUC3-eGFP cell group). B Note that pictures were taken before fixation of cells. (Top) The cells were observed under a fluorescence microscope with blue excitation. (Bottom) Pictures of phase contrast microscopy. Scale bars: 500 μm (B). Images captured at total magnification of 50× (B). The representative images show that only UMUC3-eGFP cells but not RT4 cells are able to transmigrate through the mouse bladder and proliferate in the culture wells, forming colonies.
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Universität Ulm pays non établi dans la noticeUniversité ou école supérieure
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WinnMed pays non établi dans la noticeOrganisation à but non lucratif
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Columbia University Irving Medical Center pays non établi dans la noticeÉtablissement de santé
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Université de Lille pays non établi dans la noticeUniversité ou école supérieure
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University of Tübingen pays non établi dans la noticeUniversité ou école supérieure
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University of Ulm pays non établi dans la noticeUniversité ou école supérieure
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Mayo Clinic pays non établi dans la noticeÉtablissement de santé
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Columbia University Medical Center pays non établi dans la noticeUniversité ou école supérieure
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University of Lille pays non établi dans la noticeUniversité ou école supérieure
Universität Ulm, WinnMed et Columbia University Irving Medical Center, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.