Data associated with the publication: TEAD1 condensates are transcriptionally inactive storage sites on the pericentromeric heterochromatin in cancer cells
Rattachement africain : us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
This dataset contain image data of microscopic experiments reported in the paper titled “TEAD1 condensates are transcriptionally inactive storage sites on the pericentromeric heterochromatin in cancer cells”. Abstract: TEA domain transcription factor 1 (TEAD1), a Hippo pathway transcription factor important in cellular homeostasis and development, is increasingly implicated in cancer biology. Here, we reveal a novel role for TEAD1 in organizing nuclear condensates, independent of active transcription. Using high-resolution imaging, ChIP-seq, RNA-seq and proximity-based proteomics, we demonstrate that in patient-derived renal cell carcinoma cells, TEAD1 forms micron-sized condensates by binding to the heterochromatic pericentromeric regions using its DNA-binding domain. TEAD-specific MCAT motifs selectively enrich and cluster in the pericentromeric region, specifically seeding TEAD1 condensates. TEAD1 condensates do not activate transcription but instead serve as depots for excess TEAD1, and disrupting TEAD1 condensates leads to increases in YAP/TEAD target gene expression. This organization of TEAD1 contrasts with that observed in other genomic regions of both RCC and normal kidney cells, in which TEAD1 associates with markers of active transcription. Our findings provide a mechanistic framework for TEAD1’s dual regulatory roles, offering new insights into its contribution to transcriptional dysregulation and tumor progression. Associated preprint: https://www.biorxiv.org/content/10.1101/2025.05.02.651992v2,
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Où se fait cette recherche
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Johns Hopkins University Department of Biochemistry and Molecular Biology pays non établi dans la noticeUniversité ou école supérieure
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National Cancer Institute Urologic Oncology Branch pays non établi dans la noticeOrganisme public
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California Institute of Technology Division of Chemistry and Chemical Engineering pays non établi dans la noticeUniversité ou école supérieure
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Johns Hopkins Medicine pays non établi dans la noticeÉtablissement de santé
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University of Virginia Department of Genome Sciences pays non établi dans la noticeUniversité ou école supérieure
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Johns Hopkins Bloomberg School of Public Health Department of Biochemistry and Molecular Biology pays non établi dans la noticeUniversité ou école supérieure
Department of Biochemistry and Molecular Biology — Johns Hopkins University, Urologic Oncology Branch — National Cancer Institute et Division of Chemistry and Chemical Engineering — California Institute of Technology, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.