Sex shapes CD64 expression and vaccine-induced monocytic responses
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Le résumé fourni par la source
Sex-specific differences influence vaccine-induced immunity, with females generally mounting more robust immune responses than males. While enhanced antibody production in females is well established, the cellular mechanisms underlying these differences remain elusive. Monocytes and monocyte-derived dendritic cells contribute to vaccine-induced immunity by capturing antibody-antigen immune complexes via Fc gamma receptors (FcγRs), positioning them as potential mediators of sex-biased vaccine responses. Here, we investigated sex-specific differences in FcγRI (CD64)-expressing monocytic cells in humans and mice. Using flow cytometry, we demonstrate that healthy women display higher frequencies of CD64-positive monocytes and elevated CD64 expression in peripheral blood compared with men. In mice, a similar sex-specific pattern was consistently reproduced across diverse experimental settings, including in vitro and in vivo conditions. Functional in vitro assays revealed that classical monocytes from female mice mediated stronger classical antigen presentation to CD4⁺ T cells than male-derived monocytes in a CD64-dependent manner, as reflected by increased IFNγ production. In parallel, female-derived monocytes also displayed enhanced cross-presentation to CD8⁺ T cells; however, this effect occurred independently of CD64. Following intramuscular vaccination, flow cytometric analysis demonstrated that female mice exhibited increased frequencies of CD64-positive monocytes at the injection site, accompanied by higher CD64 surface expression levels on these cells. In addition, females showed significantly greater accumulation of monocytic cells in dorsal lymph nodes compared with males. Notably, castration of male mice enhanced monocytic recruitment to muscle tissue following immunization, indicating a role for sex hormones in regulating monocytic responses to vaccination. Together, these findings identify sex-specific regulation of CD64⁺ monocytic cells as a mechanism associated with enhanced antigen presentation in females and suggest that this pathway contributes to stronger vaccine-induced immune responses. In this context, our data underscore the relevance of biological sex in shaping Fcγ receptor-mediated immune mechanisms relevant to vaccination.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Sex shapes CD64 expression and vaccine-induced monocytic responses
- Date Crossref
- 05/04/2026
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Bernhard Nocht Institute for Tropical Medicine Research Group Molecular Infection Immunology pays non établi dans la noticeStructure de recherche
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Universität Hamburg pays non établi dans la noticeUniversité ou école supérieure
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University Medical Center Hamburg-Eppendorf Institute for Infection Research and Vaccine Development (IIRVD) pays non établi dans la noticeÉtablissement de santé
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German Center for Infection Research pays non établi dans la noticeStructure de recherche
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Fraunhofer Institute for Molecular Biology and Applied Ecology pays non établi dans la noticeStructure de recherche
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Gesellschaft zur Förderung angewandter Informatik pays non établi dans la noticeOrganisation à but non lucratif
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RWTH Aachen University Institute for Molecular Biotechnology pays non établi dans la noticeUniversité ou école supérieure
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Department for Clinical Immunology of Infectious Diseases pays non établi dans la noticeÉtablissement de santé
Research Group Molecular Infection Immunology — Bernhard Nocht Institute for Tropical Medicine, Universität Hamburg et Institute for Infection Research and Vaccine Development (IIRVD) — University Medical Center Hamburg-Eppendorf, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.