Potential genotoxicity of Salvia pratensis L. aqueous extracts and their biogenic silver nanoparticles

Le résumé fourni par la source

Biosynthesis of metal nanoparticles using plant extracts is considered an eco-friendly approach with promisingbiomedical applications; however, data on their safety, particularly genotoxicity, remain limited. This study evaluated thephenolic composition and genotoxic potential of aqueous extracts from the root (SPR) and aerial parts (SPA) of Salviapratensis L. and their corresponding biogenic silver nanoparticles (SPR-AgNPs and SPA-AgNPs). Spectrophotometric methods were used to quantify total phenolic and flavonoid contents, and LC-MS analysis was employed to characterize thephenolic profiles. The genotoxic potential of the extracts and AgNPs was evaluated in human lymphocytes via the cometassay, with treatments applied at four concentrations ranging from 25 to 100 ?g/mL, either independently or in combinationwith hydrogen peroxide (H?O?). Both extracts exhibited high phenolic content, with lower flavonoid levels in SPR. LC-MSanalysis identified rosmarinic acid as the predominant compound in SPA and salvianolic acid K in SPR. Both extracts and AgNPs increased DNA damage in lymphocytes. In contrast, only the SPA extract lowered H?O?-induced DNA damage,demonstrating a pronounced protective effect. These findings indicate potential genotoxic risks associated with S. pratensisextracts and their AgNPs, while also highlighting selective protective properties depending on the plant part used.

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