Multi-tissue DNA methylation analysis to identify an appropriate surrogate tissue for a unique neurological tissue specific to spina bifida
Rattachement africain : us, bd. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: Animal models suggest a role of epigenetic mechanisms, including DNA methylation, in neural tube closure; however, studies characterizing DNA methylation profiles in nervous system tissue from humans with spina bifida are limited. In this study, we assessed DNA methylation profiles in dural tissue of infants with spina bifida, collected at the time of surgical closure of the defect, and examined whether whole blood or buccal swab are appropriate surrogate tissues, as they are more practical to collect in large-scale epidemiological studies. DNA methylation was measured in dural tissue, buccal swab, and whole blood samples collected from 27 unique infants using the Illumina Infinium MethylationEPIC BeadChip array. RESULTS: Correlation analysis for each CpG site comparing DNA methylation from all participants in dural tissue to DNA methylation in whole blood DNA or buccal swab DNA yielded 1555 statistically significant associations for the whole blood analysis and 920 significant associations for the buccal swab analysis at the Bonferroni threshold of significance. We also performed paired analysis, calculating differences between tissues within each individual and then averaging differences across individuals. After accounting for multiple hypothesis testing using the FDR adjustment, 33% of CpG sites assessed were not significantly differentially methylated between dural tissue and whole blood samples, compared to the 27% of sites not differentially methylated between dural tissue and buccal swab samples. CONCLUSIONS: These results suggest that in the absence of dural tissue, both whole blood and buccal swab samples may be considered as surrogates for dural tissue. The study warrants replication in larger groups to validate findings and may assist researchers restricted to more accessible biospecimens (i.e., blood) to further characterize epigenetic contributors to neural tube defect etiology.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Multi-tissue DNA methylation analysis to identify an appropriate surrogate tissue for a unique neurological tissue specific to spina bifida
- Date Crossref
- 05/02/2026
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Stanford University Department of Epidemiology and Population Health pays non établi dans la noticeUniversité ou école supérieure
-
National Institute of Neurosciences & Hospital pays non établi dans la noticeÉtablissement de santé
-
University of Connecticut Department of Molecular and Cell Biology pays non établi dans la noticeUniversité ou école supérieure
-
Boston Children's Hospital Department of Neurosurgery pays non établi dans la noticeÉtablissement de santé
-
Harvard University pays non établi dans la noticeUniversité ou école supérieure
-
University of Arizona Coit Center for Longevity and Neurotherapeutics pays non établi dans la noticeUniversité ou école supérieure
-
National Institute of Neurosciences and Hospital (NINS&H) Department of Paediatric Neurosurgery pays non établi dans la noticeStructure de recherche
-
Department of Clinical Neurosurgery pays non établi dans la noticeÉtablissement de santé
-
Harvard T.H. Chan School of Public Health Department of Environmental Health pays non établi dans la noticeUniversité ou école supérieure
-
R. Ken Coit College of Pharmacy Department of Pharmacology and Toxicology pays non établi dans la noticeUniversité ou école supérieure
-
Department of Neurology pays non établi dans la noticeInstitution
Department of Epidemiology and Population Health — Stanford University, National Institute of Neurosciences & Hospital et Department of Molecular and Cell Biology — University of Connecticut, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.