Preparation of Various Glycoside Hydrolase Enzyme Extracts from Durvillaea antarctica and Evaluation of the Neuroprotective Efficacy
Rattachement africain : tw. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
In this study, three distinct hydrolysates, which are designated Dur-I, Dur-II, and Dur-III, were generated from extrusion-pretreated Durvillaea antarctica biomass by applying viscozyme, cellulase, and α-amylase, respectively. Chemical analyses demonstrated distinct compositional differences among the extracts, whereas FTIR spectra verified the presence of fucose-containing sulfated polysaccharides. Furthermore, NMR analyses revealed pronounced structural variations among the extracts. To investigate neuroprotective properties of Dur-I, Dur-II, and Dur-III, rotenone (Rot) was added to SH-SY5Y cells that had been pretreated with Dur-I/II/III. Here, flow cytometry was employed to assess changes in mitochondrial membrane potential (MMP), Bcl-2 expression, cytochrome c release, caspase-9, -8, and -3 activation, as well as DNA fragmentation. The protective effect of Dur-I/II/III pretreatment of SH-SY5Y cells on the Rot-induced death process was further investigated using cell cycle and annexin V-fluorescein isothiocyanate (FITC)/PI (propidium iodide) double staining analyses. The results reveal that the Rot-induced apoptotic factors were all recovered by the pretreatment of Dur-I/II/III. Moreover, cell cycle and annexin V-FITC/PI double staining analyses also indicated that Dur-I/II/III were capable of protecting SH-SY5Y cells from Rot-induced cytotoxicity. Therefore, these Dur extracts are considered as good candidates for the prevention and treatment of neurodegeneration induced by oxidative stress.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Preparation of Various Glycoside Hydrolase Enzyme Extracts from Durvillaea antarctica and Evaluation of the Neuroprotective Efficacy
- Date Crossref
- 23/01/2026
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Yuan's General Hospital Department of Internal Medicine pays non établi dans la noticeÉtablissement de santé
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Kaohsiung Medical University Department of Internal Medicine pays non établi dans la noticeUniversité ou école supérieure
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Kaohsiung Medical University Chung-Ho Memorial Hospital pays non établi dans la noticeÉtablissement de santé
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National Taiwan Normal University Graduate Program of Nutrition Science pays non établi dans la noticeUniversité ou école supérieure
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National Kaohsiung Marine University pays non établi dans la noticeUniversité ou école supérieure
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National Kaohsiung University of Science and Technology Department of Seafood Science pays non établi dans la noticeUniversité ou école supérieure
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I-Shou University pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Medicine pays non établi dans la noticeUniversité ou école supérieure
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School of Medicine for International Students pays non établi dans la noticeUniversité ou école supérieure
Department of Internal Medicine — Yuan's General Hospital, Department of Internal Medicine — Kaohsiung Medical University et Kaohsiung Medical University Chung-Ho Memorial Hospital, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.