An Algorithm for Rapid and Low-Cost Detection of Carbapenemases Directly from Positive Blood Cultures Using an Immunochromatographic Test
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Le résumé fourni par la source
Background/Objectives: Detection of carbapenemases (KPC, OXA-48, VIM, IMP, NDM) from blood cultures (BCs) by standard methods takes 48–72 h and includes BC seeding, susceptibility testing and carbapenemase detection. Automated qPCR panels provide results in 1 h but are very costly. We aim to evaluate a low-cost and rapid immunochromatographic (IC) test directly from positive BCs using the reference method as a comparator. Methods: Ninety-one positive BCs from real-world patients and sixty-four simulated BCs were included. BC broth was treated with SDS and washed before analysis with the K.N.I.V.O. carbapenemase detection IC test. Discordant results were confirmed through the NG Carba-5 IC test and GeneXpert Carba-R qPCR test. Results: The test detected 100% of the 87 carbapenemase-producing BCs tested (sensitivity: 100% [CI95%: 95.8–100%]). However, 13 BCs generated false positive bands for NDM and/or OXA-48 (specificity: 80.8% [CI95%: 69.5–89.4%). The positive and negative predictive values were 87.0% (CI95%: 80.4–91.6%) and 100% (CI95%: 93.5–100%). Analysis of BCs providing false positive results through both confirmatory tests showed that BCs were negative for these carbapenemases. Conclusions: This is the first evaluation of the K.N.I.V.O. IC test directly from positive BCs, with a pragmatic confirmation algorithm using a second IC test or qPCR in case of NDM or OXA-48, that addresses K.N.I.V.O.’s specificity gap. The main limitation of this work is that confirmatory testing was performed only in false positives. The implementation of the K.N.I.V.O. IC test would contribute to early carbapenemase detection in BCs and is an alternative for low-resource hospitals where qPCR panels are not available.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- An Algorithm for Rapid and Low-Cost Detection of Carbapenemases Directly from Positive Blood Cultures Using an Immunochromatographic Test
- Date Crossref
- 19/12/2025
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Pontificia Universidad Católica de Chile pays non établi dans la noticeUniversité ou école supérieure
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Millennium Initiative for Collaborative Research on Bacterial Resistance pays non établi dans la noticeStructure de recherche
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Hospital Padre Hurtado pays non établi dans la noticeÉtablissement de santé
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School of Medicine Department of Clinical Laboratories pays non établi dans la noticeUniversité ou école supérieure
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Laboratory of Microbiology pays non établi dans la noticeStructure de recherche
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Multidisciplinary Initiative for Collaborative Research on Bacterial Resistance pays non établi dans la noticeInstitution
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Padre Hurtado Hospital pays non établi dans la noticeÉtablissement de santé
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Laboratory of Biochemistry pays non établi dans la noticeStructure de recherche
Pontificia Universidad Católica de Chile, Millennium Initiative for Collaborative Research on Bacterial Resistance et Hospital Padre Hurtado, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.