Resolving Incompatible Blood Cross-Matching: The Role of 37°C Water Bath in Transfusion Safety
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Le résumé fourni par la source
Background: Incompatible cross-matching at room temperature can delay critical blood transfusions. We report that red cells from patients requiring blood transfusion agglutinated after cross-matching at room temperature, but changed from agglutination to dispersion after a water bath at 37°C. The aim was to investigate whether re-cross-matching at 37°C is necessary for some patients with incompatible cross-matching. Methods: This prospective observational study analysed all incompatible cross-matched blood samples at the Blood Transfusion Department of Chifeng Municipal Hospital from 1 May 2024 to 31 July 2024. Cross-matching was performed using a fully automated system and the Polybrene method. For incompatible samples, the Polybrene method was repeated after the samples were placed in a 37°C water bath for 5 minutes. Results: Thirty patients with cross-match incompatibilities were included. Among these, agglutination was reversed after the 37°C water bath in 28 patients, indicating compatibility. Only 2 patients had true blood group incompatibility. We detail the results of one representative patient from the 28 with reversed agglutination. This patient showed agglutination using the fully automated device and the Polybrene method, but not with the saline method. After a 37°C water bath, the agglutinated erythrocytes dispersed into a single-cell state upon microscopic examination. Conclusion: For selected patients, cross-matching at 37°C can resolve false-positive agglutination, ensuring transfusion safety and timely access to blood products. This simple method can be integrated into the clinical cross-matching workflow. Plain Language Summary: Some patients experience incompatible blood cross-match results at room temperature, which can delay lifesaving transfusions.We found that for most patients in our study, this incompatibility was resolved by warming the blood sample to 37°C, allowing the transfusions to proceed safely.Incorporating a 37°C cross-matching step can help ensure that patients receive needed blood transfusions without unnecessary delay. Keywords: cross-matching incompatibilities, polybrene method, red cell aggregation, irregular antibodies, 37°C water bath
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Resolving Incompatible Blood Cross-Matching: The Role of 37°C Water Bath in Transfusion Safety
- Date Crossref
- 01/12/2025
- Éditeur
- Informa UK Limited
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Nantong University pays non établi dans la noticeUniversité ou école supérieure
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Taizhou People's Hospital Department of Clinical Laboratory pays non établi dans la noticeÉtablissement de santé
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Chifeng Municipal Hospital pays non établi dans la noticeÉtablissement de santé
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Chifeng University pays non établi dans la noticeUniversité ou école supérieure
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Baotou Medical College pays non établi dans la noticeÉtablissement de santé
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North China University of Science and Technology Precision Medicine Sequencing Center pays non établi dans la noticeUniversité ou école supérieure
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North China University of Science and Technology Affiliated Hospital pays non établi dans la noticeÉtablissement de santé
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Department of Clinical Laboratory pays non établi dans la noticeStructure de recherche
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Chifeng Clinical Medical School of Inner Mongolia Medical University pays non établi dans la noticeUniversité ou école supérieure
Nantong University, Department of Clinical Laboratory — Taizhou People's Hospital et Chifeng Municipal Hospital, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.