Upregulation of CircCSPP1, CircNRIP1, and CircSMAD2 in Breast Cancer and Their Potential as Diagnostic Biomarkers
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Le résumé fourni par la source
Background: Identifying novel diagnostic biomarkers with stable structures, such as circRNAs can improve the early detection and management of BC.Herein, we conducted this study to analyze the expression profile of four circRNAs, including circCSPP1 (hsa_circ_0001806), circNRIP1 (hsa_circ_0004771), circSMAD2 (hsa_circ_0000847), and circFOXP1 (hsa_circ_0008234) in BC.Methods: Tumor tissues and adjacent normal tissues were obtained from patients with sporadic BC.Divergent primers were designed to amplify the target transcripts using quantitative real-time PCR.The expression profiles of circRNAs were analyzed in tumor and adjacent normal tissues.Sanger sequencing was performed to confirm the back-splicing junctions of circRNAs.ROC curves were generated to assess the potential of the mentioned RNAs as diagnostic biomarkers.Results: We observed a significant upregulation of circCSPP1, circNRIP1, and circSMAD2 in tumor tissue compared to adjacent normal tissues.Among them, circCSPP1 was the most highly upregulated one in tumor samples from 39 patients.Expression of circCSPP1 was significantly higher in ER-negative, PRnegative, HER2-negative, and triple-negatives compared to ER-positive, PRpositive, HER2-positive, and non-triple-negative ones.Expression of circNRIP1 was also significantly elevated in the ER-negative and the triple-negative subgroups.These three circRNAs also displayed a desirable potential as diagnostic biomarkers.Conclusion: This is the first paper that reports the upregulation of circCSPP1 and circSMAD2 in BC and upregulation of circCSPP1 and circNRIP1 in the triplenegative subgroup.Our findings suggest that circCSPP1, circNRIP1, and circSMAD2 may serve as promising diagnostic biomarkers for BC.Identifying the downstream pathways regulated by these circRNAs could lead to the discovery of new therapeutic targets.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Upregulation of CircCSPP1, CircNRIP1, and CircSMAD2 in Breast Cancer and Their Potential as Diagnostic Biomarkers
- Date Crossref
- 01/05/2025
- Éditeur
- National Academy of Sciences of Tajikistan
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universal Scientific Education and Research Network pays non établi dans la noticeOrganisation à but non lucratif
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Children's Medical Center pays non établi dans la noticeÉtablissement de santé
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Tehran University of Medical Sciences Research Center for Immunodeficiencies pays non établi dans la noticeUniversité ou école supérieure
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Dana-Farber Cancer Institute pays non établi dans la noticeStructure de recherche
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Fasa University of Medical Sciences Department of Medical Genetics pays non établi dans la noticeUniversité ou école supérieure
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Cancer Immunology Project (CIP) pays non établi dans la noticeInstitution
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School of Medicine Department of Medical Genetics pays non établi dans la noticeUniversité ou école supérieure
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School of Public Health pays non établi dans la noticeUniversité ou école supérieure
Universal Scientific Education and Research Network, Children's Medical Center et Research Center for Immunodeficiencies — Tehran University of Medical Sciences, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.