Wound healing improvement by a multicomponent wound dressing of keratinocyte-imprinted polydimethylsiloxane substrate in a rabbit model
Rattachement africain : ir. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Wound healing is a major clinical challenge and regenerative medicine and tissue engineering suggest new ways to overcome such problems. The cell imprinting method can provide cues that regulate cell function or modulate stem cell differentiation into different lineages. In this study, the rabbit keratinocyte-imprinted polydimethylsiloxane (PDMS) substrate (KiPDMS) was made and associated with adipose-derived stem cells (ADSCs), keratinocytes, and collagen scaffold, provided various treatments. After evaluating ADSCs’ differentiation into keratinocytes by qPCR and immunocytochemistry techniques, different treatments were implanted in a rabbit ear, histopathological analysis was performed on measuring the epidermal thickness and neovascularization. The histopathological analysis revealed that treated groups increased epidermal thickness (~ 139% up to ~ 998%) and neovascularization (vessel count /mm 2 , ~ 111% to ~ 301%) compared to the control group. Collagen scaffolds stimulated re-epithelialization, and implanting ADSCs with or without other factors enhanced its effect. Adding KiPDMS significantly improved re-epithelialization and neovascularization, especially in combination with differentiated keratinocytes. The complete treatment, consisting of all cellular and non-cellular stimulating factors, associated with a significant improvements in re-epithelialization and neovascularization compared with other treatments, including collagen scaffold and some other factors alone or together. These findings suggest that each treatment may have potential applications depending on the medical conditions, particularly in situations where cellular factors are limited.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Wound healing improvement by a multicomponent wound dressing of keratinocyte-imprinted polydimethylsiloxane substrate in a rabbit model
- Date Crossref
- 27/11/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Tehran pays non établi dans la noticeUniversité ou école supérieure
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Pasteur Institute of Iran National Cell Bank Department pays non établi dans la noticeStructure de recherche
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Tehran University of Medical Sciences pays non établi dans la noticeUniversité ou école supérieure
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Amirkabir University of Technology Biomedical Engineering Department pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Veterinary Medicine Department of Surgery and Diagnostic Imaging pays non établi dans la noticeUniversité ou école supérieure
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School of Advanced Technologies in Medicine Department of Tissue Engineering pays non établi dans la noticeUniversité ou école supérieure
University of Tehran, National Cell Bank Department — Pasteur Institute of Iran et Tehran University of Medical Sciences, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.