Single-cell and bulk transcriptomics reveal Fusobacterium nucleatum promotes colorectal cancer metastasis by upregulating LAMC2 in in vitro and in vivo models
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Le résumé fourni par la source
Colorectal cancer (CRC) is one of the most prevalent malignancies worldwide. Fusobacterium nucleatum (F. nucleatum) has been strongly implicated in CRC metastasis. However, the contribution of laminin γ2 chain (LAMC2) to F. nucleatum-induced CRC metastasis remains elusive. We investigated the role of LAMC2 in F. nucleatum-induced CRC cell migration through a series of in vitro assays, including transmission electron microscopy, wound-healing, and transwell migration experiments, as well as in vivo animal models. Additionally, we explored the expression profile of LAMC2 in CRC tissues and assessed its prognostic relevance using immunohistochemistry, bulk transcriptome, and single-cell transcriptomics analyses. We found that F. nucleatum upregulates LAMC2 expression, thereby promoting CRC cell migration. Mechanistic analyses revealed that F. nucleatum downregulates hsa-miRNA-7977, releasing its transcriptional repression of LAMC2. Additionally, LAMC2 activates the FAK-PI3K-AKT signaling pathway and modulates H3K27 histone acetylation. Immunohistochemistry, together with single-cell and bulk transcriptome analyses, demonstrated that LAMC2 is highly expressed in metastatic CRC tissues, and is significantly associated with poor prognosis. Moreover, a Cox regression-based prognostic model constructed using LAMC2-associated genes exhibited robust predictive performance across multiple independent cohorts. Our findings identify LAMC2 as a key mediator of F. nucleatum-induced CRC metastasis and highlight it as both a prognostic biomarker and a potential therapeutic target.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Single-cell and bulk transcriptomics reveal Fusobacterium nucleatum promotes colorectal cancer metastasis by upregulating LAMC2 in in vitro and in vivo models
- Date Crossref
- 26/11/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Harbin Medical University Department of Colorectal Surgery pays non établi dans la noticeUniversité ou école supérieure
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Suizhou Central Hospital pays non établi dans la noticeÉtablissement de santé
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Second Affiliated Hospital of Harbin Medical University pays non établi dans la noticeÉtablissement de santé
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Sun Yat-sen University Department of Gastrointestinal Surgery pays non établi dans la noticeUniversité ou école supérieure
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First Affiliated Hospital of Harbin Medical University pays non établi dans la noticeÉtablissement de santé
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Third Affiliated Hospital of Harbin Medical University pays non établi dans la noticeÉtablissement de santé
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Hubei University of Medicine Department of Gastroenterology pays non établi dans la noticeUniversité ou école supérieure
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Cumming School of Medicine Centre for Infection and Genomics pays non établi dans la noticeUniversité ou école supérieure
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College of Pharmacy Genomics Research Center pays non établi dans la noticeUniversité ou école supérieure
Department of Colorectal Surgery — Harbin Medical University, Suizhou Central Hospital et Second Affiliated Hospital of Harbin Medical University, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.