hUCMSCs restore ovarian function via angiopoietin 1/2 rebalance in POI rats
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Le résumé fourni par la source
BACKGROUND: Premature ovarian insufficiency (POI) is often iatrogenic and the therapeutic potential of human umbilical cord derived mesenchymal stem cells (hUCMSCs) remain incompletely defined. This study aimed to evaluate the effects and underlying mechanism of hUCMSCs on ovarian function in a rat model of POI. METHODS: hUCMSCs were characterized by morphology, flow cytometry, and multi-lineage differentiation assays. POI was induced in rats using cyclophosphamide (CTX), followed by intravenous administration of hUCMSCs. Therapeutic outcomes were assessed through cell homing analysis, ovarian histopathology, and serum hormone measurements. Bioinformatic analysis identified Angiopoietin 1 (Angpt1) as a key angiogenic regulator, which was validated by western blot and qRT-PCR. Rat ovarian microvascular endothelial cells (ROMECs) were isolated and functionally assessed under CTX and hUCMSCs treatment using wound healing, tube formation, and evaluation of the Angpt1 and Angpt2 balance. RESULTS: In vivo, hUCMSCs preferentially homed to the ovarian theca layer and restored ovarian function. The Angpt1/Angpt2 ratio was markedly reduced in POI rats but significantly increased after hUCMSCs treatment, and the ratio positively correlated with CD31 expression. In vitro, CTX impaired ROMECs migration and angiogenic capacity, whereas coculture with hUCMSCs rescued these functions and restored the Angpt1/Angpt2 ratio. CONCLUSION: hUCMSCs ameliorate ovarian dysfunction in POI by modulating the Angpt1/Angpt2 axis, thereby enhancing vascular homeostasis and angiogenesis within the ovarian microenvironment. These findings provided mechanistic insights supporting the clinical translation of hUCMSCs-based therapies for POI.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- hUCMSCs restore ovarian function via angiopoietin 1/2 rebalance in POI rats
- Date Crossref
- 18/11/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Shandong University of Traditional Chinese Medicine Department of Wound Repair and Plastic Surgery pays non établi dans la noticeUniversité ou école supérieure
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Binzhou Medical University Xu Rongxiang Regenerative Medicine Research Center pays non établi dans la noticeUniversité ou école supérieure
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Shandong University of Aeronautics pays non établi dans la noticeUniversité ou école supérieure
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Affiliated Hospital of Shandong University of Traditional Chinese Medicine pays non établi dans la noticeÉtablissement de santé
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The First Clinical Medical College pays non établi dans la noticeUniversité ou école supérieure
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The Second Clinical Medical College pays non établi dans la noticeUniversité ou école supérieure
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College of Basic Medicine Department of Histology and Embryology pays non établi dans la noticeUniversité ou école supérieure
Department of Wound Repair and Plastic Surgery — Shandong University of Traditional Chinese Medicine, Xu Rongxiang Regenerative Medicine Research Center — Binzhou Medical University et Shandong University of Aeronautics, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.