Innovative Application of a Multifunctional Sucrose–Gelatin Hydrogel Matrix in Desorption Electrospray Ionization-Mass Spectrometry Imaging
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Le résumé fourni par la source
Desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) is among the most powerful techniques for visualizing the spatial distribution of small organic molecules, particularly lipids, on tissue surfaces. Conventional DESI-MSI analysis typically involves sectioning fresh-frozen tissues or, less commonly, embedding samples in matrices specifically formulated to preserve the tissue integrity for multifunctional analyses. In this study, we present an optimized sucrose-gelatin hydrogel matrix compatible with DESI-MSI, using mouse brain tissue as a model system. The method involves low-temperature embedding of frozen specimens into the hydrogel matrix, followed by snap-freezing at -160 °C. This matrix formulation ensures minimal background interference and prevents metabolite delocalization, thereby preserving the native molecular composition of the tissue. Notably, sucrose-derived adduct ions restricted to the embedding medium serve as stable internal reference signals in both positive and negative ionization modes. These signals enable continuous lock-mass correction throughout acquisition, offering a new solution to the unresolved challenge for accurate mass-based measurements in DESI-MSI without an infusion of exogenous calibration standards. Complementary DESI-MS/MS analyses further facilitate confident lipid identification and resolve structural ambiguities. Moreover, the sucrose-gelatin embedding medium provides excellent preservation of tissue morphology and antigenicity, supporting subsequent histological and immunohistochemical analyses. Overall, this sucrose-based hydrogel embedding protocol offers a robust, reproducible, and multimodal platform for molecular tissue imaging by DESI-MSI, especially in delicate biological specimens with broad translational potential across preclinical and clinical research domains.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Innovative Application of a Multifunctional Sucrose–Gelatin Hydrogel Matrix in Desorption Electrospray Ionization-Mass Spectrometry Imaging
- Date Crossref
- 07/11/2025
- Éditeur
- American Chemical Society (ACS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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National Research Council pays non établi dans la noticeOrganisation à but non lucratif
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Istituto Nazionale di Fisica Nucleare pays non établi dans la noticeStructure de recherche
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University of Naples Federico II pays non établi dans la noticeUniversité ou école supérieure
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Institute of Genetics and Biophysics pays non établi dans la noticeStructure de recherche
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Institute of Biomolecular Chemistry (ICB) pays non établi dans la noticeStructure de recherche
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University of Napoli “Federico II” Department of Biology pays non établi dans la noticeUniversité ou école supérieure
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University of Napoli "Federico II" Department of Biology pays non établi dans la noticeUniversité ou école supérieure
National Research Council, Istituto Nazionale di Fisica Nucleare et University of Naples Federico II, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.