Spinal Cord Injury in Enterovirus D68 Infection: Mechanisms and Pathophysiology in a Mouse Model
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Le résumé fourni par la source
Enterovirus D68 (EV-D68) is a significant global pathogen associated with severe respiratory infections and acute flaccid myelitis in children. Currently, there are no vaccines or antiviral drugs available for EV-D68, and a robust model to elucidate the pathogenesis of EV-D68 and evaluate treatment methods is lacking. We developed a mouse-adapted EV-D68 strain that caused progressive limb paralysis after intramuscular inoculation in 7-day-old mice. Viral load analysis showed that the skeletal muscle and spinal cord had the highest titers and most severe injuries. RNA sequencing of the infected muscle, brain, spinal cord, and lung tissues revealed differentially expressed genes (DEGs) associated with viral infection and pathogenesis. DEGs were significantly enriched in various pathways associated with antiviral immunity, interferon responses, and cytokine signaling. In the spinal cord, DEGs highlighted mitochondrial dysfunction and oxidative stress as crucial contributors to neural damage. Flow cytometry analysis of spinal cord cells showed that EV-D68 activates the immune system, leading to systemic inflammation and significant increases in CD8+ and CD4+ T cells, but limited neutrophil and monocyte infiltration. This mouse model provides a valuable tool for studying EV-D68 pathogenesis and evaluating antiviral and vaccine efficacy, thereby advancing the understanding of its neuropathological mechanisms. Importance: We developed a novel mouse model of EV-D68 that provides a valuable tool for studying its pathogenesis and evaluating antiviral and vaccine efficacy, deepening the understanding of its neuropathological basis.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Spinal Cord Injury in Enterovirus D68 Infection: Mechanisms and Pathophysiology in a Mouse Model
- Date Crossref
- 06/11/2025
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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National Health and Family Planning Commission pays non établi dans la noticeOrganisme public
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National Institute for Viral Disease Control and Prevention pays non établi dans la noticeOrganisme public
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National Health Commission Key Laboratory of Microbial Genomics pays non établi dans la noticeStructure de recherche
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National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases (NITFID) pays non établi dans la noticeStructure de recherche
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National Polio Laboratory pays non établi dans la noticeStructure de recherche
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World Health Organization Polio Reference Laboratory for the Western Pacific Region pays non établi dans la noticeStructure de recherche
National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention et National Health Commission Key Laboratory of Microbial Genomics, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.