Hypothermia after Cardiac Arrest in Large Animals (HACA-LA): a randomized controlled experimental study
Rattachement africain : se, cz, at, cn, fr, us, gb, in. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: Induced hypothermia after cardiac arrest is neuroprotective in several animal models of cardiac arrest, but few high-quality studies have been conducted in larger animals. Recent clinical trials have questioned the beneficial effects of post-ischemic hypothermia. This study investigated whether immediate cooling or a 2-h delay in cooling to 33 °C after cardiac arrest was neuroprotective compared to controlled normothermia in large animals. METHODS: Young adult female swine were anesthetized and kept at normothermia (38 °C). All animals were subject to 10 min of cardiac arrest by ventricular fibrillation, followed by 4 min of cardiopulmonary resuscitation, before the first countershock. At 10 min of return of spontaneous circulation (ROSC), animals were included and randomized to receive immediate hypothermia (33 °C), 2-h delayed hypothermia (33 °C), or normothermia for 30 h, including both cooling and rewarming time. Animals were extubated and assessed for 7 days. The primary outcome was brain histopathology using a modified Histology Damage Score. Secondary outcomes were neurocognitive testing, neurologic deficit score, and biomarkers of brain injury. RESULTS: Among 42 animals, 33 were included; 11 in each arm, 23 survived until day 7. The modified Histology Damage Score was not significantly different between groups (p = 0.29). Neither neurocognitive testing nor neurologic deficit scores showed significant differences between the groups (p = 0.11 and p = 0.67, respectively). Neurofilament light chain (NfL) levels were significantly lower in the immediate hypothermia group at 48 h and on day 7 compared to the normothermia group (p = 0.0087, p = 0.012), but not in the delayed hypothermia group (p = 0.075, p = 0.33). CONCLUSION: Our experimental model in large swine showed no neuropathological or functional protective effect of induced hypothermia after cardiac arrest, but NfL levels were lower in animals receiving immediately induced hypothermia, suggesting mitigation of neuronal injury. TRIAL REGISTRY: Preclinicaltrials.eu (PCTE0000272), published 2021-11-03.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Hypothermia after Cardiac Arrest in Large Animals (HACA-LA): a randomized controlled experimental study
- Date Crossref
- 22/10/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Lund University Department of Clinical Sciences pays non établi dans la noticeUniversité ou école supérieure
-
Skåne University Hospital Department of Intensive and Perioperative Care pays non établi dans la noticeÉtablissement de santé
-
Charles University pays non établi dans la noticeUniversité ou école supérieure
-
Czech Academy of Sciences pays non établi dans la noticeStructure de recherche
-
General University Hospital in Prague Third Department of Internal Medicine pays non établi dans la noticeOrganisme public
-
Medical University of Vienna Department of Emergency Medicine pays non établi dans la noticeUniversité ou école supérieure
-
University of Science and Technology of China Neurodegenerative Disorder Research Center pays non établi dans la noticeUniversité ou école supérieure
-
Sahlgrenska University Hospital Clinical Neurochemistry Lab pays non établi dans la noticeÉtablissement de santé
-
Sorbonne Université pays non établi dans la noticeUniversité ou école supérieure
-
Institut des Sciences Moléculaires pays non établi dans la noticeStructure de recherche
-
Pitié-Salpêtrière Hospital pays non établi dans la noticeÉtablissement de santé
-
Institut du Cerveau pays non établi dans la noticeStructure de recherche
Department of Clinical Sciences — Lund University, Department of Intensive and Perioperative Care — Skåne University Hospital et Charles University, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.