Safety evaluation of the food enzyme d‐psicose 3‐epimerase from the genetically modified Escherichia coli strain K‐12 W3110‐TKO CDX‐088

Résumé fourni par la source

strain K-12 W3110-TKO CDX-088 by Tate & Lyle Ingredients France SAS. The production strain of the food enzyme contains several copies of a known antimicrobial resistance gene. However, based on the absence of viable cells and DNA from the production organism in the food enzyme, this is not considered to be a risk. The food enzyme is intended to be used in the processing of sugars for the production of specialty carbohydrates (d-allulose). Since the Panel considered that residual amounts of food enzyme-total organic solids are removed during the production of d-allulose, dietary exposure was not calculated and toxicological testing was not needed. A search for the homology of the amino acid sequence of the d-psicose 3-epimerase to known allergens was made and no match was found. The Panel considered that a risk of allergic reactions upon dietary exposure to the food enzyme cannot be excluded, but that the likelihood is low. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns under the intended conditions of use.