Hierarchical wrinkle pattern drives tenogenic differentiation from human mesenchymal stem cells

Le résumé fourni par la source

Stem cells possess the unique abilities to self-renew and differentiate into various cell types, making them invaluable in tissue engineering and regenerative medicine. This study explores the behavior of human bone marrow-derived mesenchymal stem cells (hMSCs) on poly(dimethylsiloxane) (PDMS) substrates with hierarchical wrinkle patterns. These bio-inspired patterns were created using the surface instability of gold-coated elastomer bilayers. The results indicate that the hierarchical wrinkles not only promote cell alignment but also up-regulate tenogenic differentiation markers without chemical induction. The cells exhibited increased expression of tenocyte markers ( Mkx , and Col1 ) and decreased expression of osteoblast ( Alp , and Opn ) and chondrocyte ( Sox9 ) markers. This bio-inspired substrate design, mimicking natural extracellular matrix structures, provides a promising approach for developing functional tissue constructs and advancing stem cell-based regenerative therapies. The study underscores the importance of substrate topography in directing stem cell fate, highlighting its potential in mechanobiological applications and tissue engineering.

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