In Vivo Characterization and Tissue Tropism of a Wild-Type Yellow Fever Virus Isolate from the 2017–2018 Brazilian Outbreak in C57BL/6 IFNAR1−/− Mice
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Le résumé fourni par la source
Yellow fever remains a significant public health concern in endemic regions of South America and Africa, where periodic outbreaks continue to challenge surveillance and control efforts. Despite the widespread use of vaccines and historical YFV strains in experimental settings, there is limited information on the pathogenic behavior of contemporary wild-type isolates in animal models. To address this gap, this study aimed to develop and characterize a murine model infected with a wild-type YFV strain isolated in 2018, from Brazil’s largest sylvatic outbreak in decades. In this study, four-week-old male and female C57BL/6 IFNAR1−/− mice were subcutaneously infected with WT YFV. Mice exhibited a nearly 50% survival rate and developed several clinical signs. Viral loads were assessed in serum and some tissues, collected either upon euthanasia of moribund animals or at the end point. YFV RNA was detected in all sampled tissues and serum. Infectious viral particles were identified in the brains of both sexes and in the testis. No statistically significant differences were observed between males and females in survival, clinical signs, or viral loads. Altogether, this study provides a robust and reproducible murine model for wild-type YFV infection, offering a valuable platform for investigating viral pathogenesis, host responses, and potential therapeutic interventions.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- In Vivo Characterization and Tissue Tropism of a Wild-Type Yellow Fever Virus Isolate from the 2017–2018 Brazilian Outbreak in C57BL/6 IFNAR1−/− Mice
- Date Crossref
- 29/09/2025
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universidade Federal de Minas Gerais pays non établi dans la noticeUniversité ou école supérieure
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Fundação Oswaldo Cruz pays non établi dans la noticeStructure de recherche
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Crozet BioPharma (United States) pays non établi dans la noticeEntreprise
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Stanford University Department of Pediatrics pays non établi dans la noticeUniversité ou école supérieure
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Federal University of Minas Gerais (UFMG) Laboratório de Vírus—Institute of Biological Sciences (ICB) pays non établi dans la noticeUniversité ou école supérieure
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Oswaldo Cruz Foundation (FIOCRUZ)—René Rachou Institute pays non établi dans la noticeStructure de recherche
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Crozet BioPharma LLC pays non établi dans la noticeInstitution
Universidade Federal de Minas Gerais, Fundação Oswaldo Cruz et Crozet BioPharma (United States), avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.