Living/controlled supramolecular protein polymerization
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Le résumé fourni par la source
With the learning from living protein polymerization in nature, achieving living/controlled supramolecular assembly of biopolymers such as proteins in vitro is a longstanding challenge for material design. Herein, we provide a thiol-regulated interfacial protein aggregation (TRIPA) for unfolded protein systems with typical living polymerization features. By triggering globule proteins into the unfolded state through a reversible exchange reaction of disulfide bonds and sulfhydryl agents (R-SH), protein monomers are partially unfolded and assembled at the air-water/solid-water interface (AWI/SWI) through the entropy-driven adsorption and conformation transition. The process could be well repeated over time to form a two-dimensional (2D) nanofilm at the interface by following a pathway of monomer-oligomer-2D assembly. Similar to living polymerization, the film thickness exhibited a linear increase with the assembly conversion ratio of the monomers. With the stepwise addition of native protein into the reaction system, the thickness periodically increased in a linear manner. Such living/controlled supramolecular polymerization (LCSP) of protein at the interface leads to the synthesis of a nanofilm with well-defined flat morphology, ultrahigh modulus, and nano- to macroscale controlled thickness. The resultant protein nanofilm could then attach onto a variety of flexible and rigid material surfaces to produce a stable structural color coating. Compelling evidence in the present work thus underlines a demonstration of LCSP of biopolymers in vitro. It may hold a solid impact by opening a window for living/controlled polymerization of versatile biospecies such as proteins, saccharides, nucleic acids, and cells.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Living/controlled supramolecular protein polymerization
- Date Crossref
- 30/09/2025
- Éditeur
- National Academy of Sciences
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Shaanxi Normal University pays non établi dans la noticeUniversité ou école supérieure
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Chinese Academy of Sciences pays non établi dans la noticeOrganisme public
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Changchun Institute of Applied Chemistry pays non établi dans la noticeStructure de recherche
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State Key Laboratory of Polymer Physics and Chemistry pays non établi dans la noticeStructure de recherche
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Jianghan University pays non établi dans la noticeUniversité ou école supérieure
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School of Chemistry and Chemical Engineering Key Laboratory of Applied Surface and Colloid Chemistry pays non établi dans la noticeUniversité ou école supérieure
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College of Life Science pays non établi dans la noticeUniversité ou école supérieure
Shaanxi Normal University, Chinese Academy of Sciences et Changchun Institute of Applied Chemistry, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.