Dual Gatekeepers-Driven Signal Amplification Strategy for Precise Detection and Modulation of Ovarian Cancer Exosome Subtypes
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Le résumé fourni par la source
Early detection of ovarian cancer remains a significant challenge due to the lack of symptoms in its early stages and the overlap in protein expression patterns between malignant and benign conditions. In this study, we introduce a dual gatekeepers-driven signal amplification strategy for the highly sensitive detection and precise modulation of ovarian cancer-derived exosome subtypes. By employing CA125 and carcinoembryonic antigen (CEA) aptamers as dual gatekeepers, this strategy selectively activates functional regions on exosome membranes, triggering the opening of hairpin DNA structures (HP) upon recognition of specific protein patterns. The opened HP then initiate an exonuclease III-powered DNA walking system and nucleic acid-stabilized Ag 0 nanoclusters (AgNCs), significantly amplifying the detection sensitivity. This approach not only enables the specific differentiation of malignant exosomes from benign ones but also facilitates the distinction between early and late-stage tumors. Moreover, it regulates the interaction between ovarian cancer-derived exosomes and target cells, promoting the exchange of biological information and material transfer. This innovative strategy serves as a powerful tool for early tumor detection while offering valuable insights into the interactions between exosome subtypes and tumor cells. Its application establishes a strong theoretical foundation for investigating the roles of exosomes in intercellular communication, immune evasion, and other biological processes, with far-reaching implications for cancer immunotherapy and related research.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Dual Gatekeepers-Driven Signal Amplification Strategy for Precise Detection and Modulation of Ovarian Cancer Exosome Subtypes
- Date Crossref
- 19/09/2025
- Éditeur
- American Chemical Society (ACS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Shandong Provincial QianFoShan Hospital pays non établi dans la noticeÉtablissement de santé
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Shandong First Medical University Medical Science and Technology Innovation Center pays non établi dans la noticeUniversité ou école supérieure
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Shandong Provincial Hospital pays non établi dans la noticeÉtablissement de santé
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Chinese Academy of Sciences pays non établi dans la noticeOrganisme public
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Institute of Zoology pays non établi dans la noticeStructure de recherche
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Medical Science and Technology Innovation Center pays non établi dans la noticeInstitution
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Laboratory of Stem Cell and Reproductive Biology pays non établi dans la noticeStructure de recherche
Shandong Provincial QianFoShan Hospital, Medical Science and Technology Innovation Center — Shandong First Medical University et Shandong Provincial Hospital, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.