Systematic changes in intestinal mucosal barrier function and related aspects of laying hens during the aging process

Le résumé fourni par la source

1. Reveals systematic age-related decline in intestinal barrier integrity in laying hens, characterized by villus shortening, goblet cell loss, increased permeability, and tight junction disruption. 2. Identifies Treg-macrophage immune imbalance as a key driver of intestinal inflammaging, evidenced by decreased Treg-derived IL-10 and elevated macrophage-derived TNF-α promoting epithelial senescence. Aging leads to diminished gut function, manifesting as gut dysbiosis, chronic inflammation, and increased intestinal permeability at both the organ and cellular levels. However, few studies have focused on the systematic changes in the intestinal mucosal barrier function of laying hens during aging process. Samples were collected from the laying hens at the 40th, 70th, and 100th weeks of age to assess related indexes. As laying hens age, their feed intake gradually decreases, and both egg quality and production performance decline. Notably, expression levels of intestinal cellular senescence markers increased as the age of laying hens increased. Aging is related to changes in intestinal mucosal barrier function, such as shortened ileal villi, reduced goblet cell numbers, heightened intestinal permeability, and disrupted immune homeostasis. Further studies showed that regulatory T (Treg) cell-derived interleukin (IL)-10 decreases. Moreover, macrophage-derived tumor necrosis factor (TNF)-α was elevated and promoted senescence in intestinal epithelial cells of chicken organoids. Critically, declining feed intake likely exacerbates intestinal dysfunction by limiting nutrient availability for barrier maintenance and immune regulation. This study elucidates age-related alterations in intestinal mucosal barrier function and highlights the role of Treg cell and macrophage homeostasis in intestinal inflammaging in laying hens.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.