Ganglioside Profiling Uncovers Distinct Patterns in High-Risk Neuroblastoma
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Le résumé fourni par la source
High-risk (HR) neuroblastoma (NBL) patients often receive standardized treatment despite wide variations in clinical outcomes, underscoring the need for improved stratification tools. A distinguishing feature of NBL is the patient-specific expression of gangliosides (GGs), particularly GD2, which may serve as biomarkers. We analyzed GG profiles in 18 patient-derived tumors and 11 NBL cell lines using thin-layer chromatography and mass spectrometry. Expression of 0-, a-, and b-series GGs was examined and correlated with clinical risk, outcome, and gene expression data. Low-risk (LR) tumors expressed higher levels of complex b-series GGs. In HR tumors, five GG profiles (A–E) were identified. Profile A featured complex b-series GGs; B showed GD2 dominance; C showed synthesis arrest at GM3 or GD3 due to low expression of the GM2/GD2 synthase, encoded by the B4GALNT1 gene; D included complex a- and b-series GGs; and E was marked by GM2 and GD1a prevalence. B4GALNT1 expression served as a prognostic marker. Relapsed tumors following anti-GD2 therapy typically exhibited reduced GD2 levels, except for one profile A tumor that displayed a ceramide anchor shorter than those found in LR tumors. Astonishingly, the ceramide anchor composition of GD2 itself appears to separate LR and HR NBL, hinting at a role of ceramide synthases in NBL biology. All cell lines expressed GM2, but exhibited very low levels of complex b-series GGs. Profile C was found only in cell lines of the mesenchymal subtype. These findings support further investigation of GG composition and associated enzyme expression as potential biomarkers for risk stratification and treatment response in NBL.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Ganglioside Profiling Uncovers Distinct Patterns in High-Risk Neuroblastoma
- Date Crossref
- 29/08/2025
- Éditeur
- MDPI AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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German Cancer Research Center Germany pays non établi dans la noticeStructure de recherche
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Johannes Gutenberg University Mainz Department of Pediatric Hematology/Oncology pays non établi dans la noticeUniversité ou école supérieure
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Heidelberg University pays non établi dans la noticeUniversité ou école supérieure
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University Medical Center of the Johannes Gutenberg University Mainz pays non établi dans la noticeÉtablissement de santé
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Helmholtz Institute Mainz pays non établi dans la noticeOrganisme public
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Helmholtz Institute for Translational Oncology Mainz pays non établi dans la noticeÉtablissement de santé
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Istituto Giannina Gaslini pays non établi dans la noticeÉtablissement de santé
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University Cancer Center (UCT) pays non établi dans la noticeUniversité ou école supérieure
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Laboratory of Experimental Therapies in Oncology pays non établi dans la noticeStructure de recherche
Germany — German Cancer Research Center, Department of Pediatric Hematology/Oncology — Johannes Gutenberg University Mainz et Heidelberg University, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.