Insights into the relationship between nasal bacterial composition and susceptibility to early-life respiratory disease: a pilot observational study
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Le résumé fourni par la source
Abstract Background Early-life susceptibility to viral respiratory infections is associated with long-term respiratory morbidity in children. Currently, no reliable tools exist to predict susceptibility to these infections. Because the endogenous microbiota can influence pathogen virulence and airway inflammation, it represents a potential target for prevention. In this pilot study, we hypothesised that distinct nasal microbial communities are associated with susceptibility to viral respiratory infections and wheezing outcomes during the first year of life. Methods We analyzed 90 nasal swabs from 55 infants enrolled in the AERIAL study, representing asymptomatic samples collected at scheduled visits (∼4 months) and symptomatic samples. Bacterial profiling was done blind to clinical data using full-length 16S rRNA sequencing, and bacterial load was quantified using a pan-bacterial TaqMan® assay. Results Bacterial diversity was similar between asymptomatic and symptomatic swabs, with community composition differences largely driven by inter-individual variation. In paired samples, symptomatic swabs showed reduced diversity but no change in bacterial load. Virus-bacteria interactions were observed in rhinovirus-positive swabs, but not SARS-CoV-2-positive swabs. Two nasal endotypes were identified, dominated by Moraxella or Streptococcus and differing in alpha diversity. Endotypes were associated with age at wheeze onset, and their relationship with wheezing episode rates showed a suggestive sex-dependent pattern that warrants further investigation. Conclusion Our pilot data suggest that the nasal microbiota might shape early wheezing outcomes in a sex-dependent manner, and highlight the value of longitudinal studies for clarifying how host-bacteria-virus interactions in early life. What is tested Whether distinct nasal microbiota endotypes in infancy are associated with later respiratory phenotypes, particularly wheeze. What the study showed Two endotypes were identified. Moraxella-dominated and Streptococcus-dominated, with differences in alpha diversity and associations with age at wheeze onset and wheezing rates. The significance and clinical impact of the research outcomes Nasal microbiota endotypes may help identify infants at risk of wheeze and support early risk stratification, pending validation in larger cohorts.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Insights into the relationship between nasal bacterial composition and susceptibility to early-life respiratory disease: a pilot observational study
- Date Crossref
- 19/08/2025
- Éditeur
- openRxiv
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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The Kids Research Institute Australia pays non établi dans la noticeOrganisation à but non lucratif
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The University of Western Australia UWA Centre for Child Health Research pays non établi dans la noticeUniversité ou école supérieure
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Curtin University Curtin Health and Innovation Research Institute (CHIRI) pays non établi dans la noticeUniversité ou école supérieure
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Australian Research Council pays non établi dans la noticeOrganisme public
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Friedrich Schiller University Jena pays non établi dans la noticeUniversité ou école supérieure
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Princess Margaret Hospital for Children pays non établi dans la noticeÉtablissement de santé
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Harry Perkins Institute of Medical Research pays non établi dans la noticeÉtablissement de santé
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Edith Cowan University pays non établi dans la noticeUniversité ou école supérieure
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Government of Western Australia Department of Health pays non établi dans la noticeOrganisme public
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University of Arizona Asthma and Airway Disease Research Center pays non établi dans la noticeUniversité ou école supérieure
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University of Maryland Nova Institute for Health pays non établi dans la noticeUniversité ou école supérieure
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Telethon Kids Institute pays non établi dans la noticeStructure de recherche
The Kids Research Institute Australia, UWA Centre for Child Health Research — The University of Western Australia et Curtin Health and Innovation Research Institute (CHIRI) — Curtin University, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.