Single-cell Raman spectroscopic analysis of bacteroids in soybean nodules to observe the relationship between biomolecular constituents and symbiotic nitrogen fixation activity
Rattachement africain : jp. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Nitrogen fixation in soybean occurs as a result of symbiosis between the plant and rhizobia in the nodules. This process allows both the plant and the symbiont to acquire vital nutrition. To fully understand the symbiosis, many researchers have attempted to attain a deeper interpretation of the biomolecular behavior or enhance the nitrogen fixation activity of bacteroids. However, most studies have focused on forward and reverse genetics approaches to evaluate the contribution of a particular gene/enzyme in nitrogen fixation. Few studies have observed the bacteroids' overall biomolecular behavior in the nodules. Thus, we grew soybean plants and recorded acetylene reduction assay (ARA) results at several growth stages. Simultaneously, we analyzed the biomolecular compounds in the bacteroids in the nodules at the single-cell level by Raman microspectroscopy. Random forest regression, a machine learning method, was applied to discover the biomolecular contribution to the ARA, as it predicted ARA results with high accuracy. Polyhydroxybutyrate (PHB) biopolymer significantly contributed to predicting ARA results, suggesting its potential relevance in symbiotic nitrogen fixation in soybean. Further studies related to PHB behavior will lead to a deeper understanding of symbiotic nitrogen fixation and may help achieve better control of this process to increase crop yields.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Single-cell Raman spectroscopic analysis of bacteroids in soybean nodules to observe the relationship between biomolecular constituents and symbiotic nitrogen fixation activity
- Date Crossref
- 25/09/2025
- Éditeur
- Japanese Society for Plant Cell and Molecular Biology
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Waseda University Research Organization for Nano and Life Innovation pays non établi dans la noticeUniversité ou école supérieure
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Tokyo University of Agriculture and Technology Institute of Agriculture pays non établi dans la noticeUniversité ou école supérieure
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National Institute of Advanced Industrial Science and Technology pays non établi dans la noticeOrganisme public
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Kyushu University pays non établi dans la noticeUniversité ou école supérieure
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Graduate School of Advanced Science and Engineering Department of Advanced Science and Engineering pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Agriculture pays non établi dans la noticeUniversité ou école supérieure
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Biomanufacturing and Process Research Center (BPRC) pays non établi dans la noticeStructure de recherche
Research Organization for Nano and Life Innovation — Waseda University, Institute of Agriculture — Tokyo University of Agriculture and Technology et National Institute of Advanced Industrial Science and Technology, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.