Transcriptome sequencing showed the differential expression of circRNAs in human pulmonary microvascular endothelial cells in acute respiratory distress syndrome
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Le résumé fourni par la source
Background: Acute respiratory distress syndrome (ARDS) is characterized by elevated pulmonary microvascular permeability; however, the role of circular RNAs (circRNAs) in this process remains unclear. Our study aims to discover the mechanism underlying the role of circRNA in pulmonary microvascular permeability in ARDS. Methods: model of ARDS using cultured human pulmonary microvascular endothelial cells (HPMECs) and lipopolysaccharide challenge. Genome sequencing revealed significant differences among the cells in the expression of circRNA. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of the target genes were conducted. A circRNA-microRNA (miRNA)-messenger RNA (mRNA) competitive endogenous RNA (ceRNA) network was constructed. The GO enrichment analysis of the target genes in the ceRNA network was analyzed. Results: The genome sequencing results identified 379 significantly upregulated circRNAs and 448 significantly downregulated circRNAs. The 10 circRNAs with the greatest degree of upregulation and the 10 circRNAs with the greatest degree of downregulation were identified. The GO enrichment analysis results indicated that differential circRNA expression may mediate the cellular response to DNA damage, including DNA repair. The KEGG analysis results indicated that the mechanism by which differential circRNA expression exerts these effects may involve the mitogen-activated protein kinases (MAPK) signaling pathway. The GO enrichment analysis of the target genes in the ceRNA network showed that the circRNAs were mainly involved in the fluid shear stress response, angiogenesis regulation, vascular development, and cell adhesion. Conclusions: The differential expression of circRNAs may play an important role in ARDS, especially in the control of HPMEC permeability. The circRNAs that were shown to have differential expression in response to vascular development and shear stress response could be used as biomarkers for the early prediction of ARDS disease and potential future therapeutic targets.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Transcriptome sequencing showed the differential expression of circRNAs in human pulmonary microvascular endothelial cells in acute respiratory distress syndrome
- Date Crossref
- 01/06/2025
- Éditeur
- AME Publishing Company
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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People 's Liberation Army 451 Hospital pays non établi dans la noticeÉtablissement de santé
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Xi'an Medical University pays non établi dans la noticeUniversité ou école supérieure
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Affiliated Hospital of Shaanxi University of Chinese Medicine Department of Respiratory pays non établi dans la noticeÉtablissement de santé
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Shaanxi University of Chinese Medicine pays non établi dans la noticeUniversité ou école supérieure
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Texas A&M University Department of Medicine & Pharmacology pays non établi dans la noticeUniversité ou école supérieure
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Baylor University Medical Center Department of Thoracic Surgery pays non établi dans la noticeÉtablissement de santé
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The Second Affiliated Hospital of Air Force Medical University Department of Respiratory pays non établi dans la noticeUniversité ou école supérieure
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Graduate Faculty pays non établi dans la noticeUniversité ou école supérieure
People 's Liberation Army 451 Hospital, Xi'an Medical University et Department of Respiratory — Affiliated Hospital of Shaanxi University of Chinese Medicine, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.