Optimized multiplex PCR-NGS for comprehensive HLA genotyping in Chinese populations: resolving ambiguities at high resolution
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Le résumé fourni par la source
Introduction: Accurate human leukocyte antigen (HLA) genotyping is critical for organ transplantation to ensure donor-recipient compatibility. Conventional methods, such as sequence-based typing (SBT), often face challenges in resolving allelic ambiguities, particularly in highly polymorphic regions of HLA loci. Therefore, this study aimed to develop 6 locus multiplex primers combined with Next-generation sequencing NGS for high-resolution of long sequenceshigh-resolution sequencing, focusing on improving sequencing depth and reducing costs. Methods: Multiplex PCR primers targeting HLA-A, -B, -C, -DPB1, -DQB1, -DRB1 loci were designed using high-frequency alleles from public databases. PThe primers were optimized using as reference the sequencing depth across loci. The method was validated using SBT and probe capture‑based targeted next‑generation sequencing to evaluate its approach accuracy. Moreover, 770 samples from Chinese population were further studied to verify the allele frequency adding information about HLA types of this population. Results: The optimized multiplex PCR-NGS sequencing showed depths within athe target range of 100-1000 with high accuracy determined in the 2ndtwo-digit ,and 4thfour-digit and six-digit HLA typing, with a reliability of ≥ 98%, ≥ 95% and ≥ 95% respectively in both methods. Discussion: Allele digits in the HLA-class I and II loci. However, in the 6th digit of HLA-C, -DQB1, and -DRB1 the accuracy was 94.74%. The developed multiplex PCR-NGS method offers a reliable, cost-effective approach for high-resolution HLA genotyping, and may be particularly suitable for clinical studies, especially in donor-recipient matching during organ transplantation.
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DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Optimized multiplex PCR-NGS for comprehensive HLA genotyping in Chinese populations: resolving ambiguities at high resolution
- Date Crossref
- 26/06/2025
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Jiangsu University pays non établi dans la noticeUniversité ou école supérieure
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Xuzhou Medical College pays non établi dans la noticeUniversité ou école supérieure
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Affiliated Hospital of Xuzhou Medical College pays non établi dans la noticeÉtablissement de santé
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Viva Biotech (China) pays non établi dans la noticeEntreprise
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The Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University Nephrology Department pays non établi dans la noticeUniversité ou école supérieure
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School of Life Sciences pays non établi dans la noticeUniversité ou école supérieure
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Ltd Co. pays non établi dans la noticeEntreprise
Jiangsu University, Xuzhou Medical College et Affiliated Hospital of Xuzhou Medical College, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.