Induced Sputum Transcriptomics Profile and Serum C3 are Associated with Asthma Severity
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Le résumé fourni par la source
Rational: Asthma severity assessment is essential for asthma management. Transcriptomics contributes substantially to asthma pathogenesis. Then, this study aimed to explore asthma severity-associated transcriptomics profile and promising biomarkers for asthma severity prediction. Methods: In discovery cohort, induced sputum cells from 3 non-severe and 3 severe asthma patients were collected and analyzed using RNA-seq. Multivariate analysis was performed to explore asthma severity-associated transcriptomics profile and differential expressed genes (DEGs). The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) were used for pathway enrichment analysis. Subsequently, based on the previous study and clinical experience, the mRNA expressions of 6 overlapped asthma severity-associated DEGs and C3 in induced sputum cells and serum C3 were verified in validation cohort. Results: Distinct asthma severity-associated transcriptomics profile was identified in induced sputum cells in discovery cohort. Then, 345 DEGs were found, of which 38 terms and 32 pathways were enriched using GO and KEGG, respectively. In validation cohort, the mRNA expressions of ZNF331, CD163, MACC1, ADAMTS2 , and C3 were increased, and RYR1 and NRXN3 were decreased in induced sputum cells in severe asthma. Meanwhile, the AUC of ROC was 0.890 for serum C3 in asthma severity prediction, with the best cut-off of 1.272 g/L. Conclusion: Collectively, this study provides the first identification of the association between induced sputum cells transcriptomics profile and asthma severity, indicating the potential value of transcriptomics for asthma management. The study also reveals the promising value of serum C3 for predicting asthma severity in clinical practice. Keywords: asthma severity, induced sputum, RNA-seq, serum C3, ACT scores, FeNO
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Induced Sputum Transcriptomics Profile and Serum C3 are Associated with Asthma Severity
- Date Crossref
- 01/06/2025
- Éditeur
- Informa UK Limited
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Suizhou Central Hospital pays non établi dans la noticeÉtablissement de santé
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Fudan University Department of Respiratory Medicine and Critical Care Medicine pays non établi dans la noticeUniversité ou école supérieure
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Zhongshan Hospital pays non établi dans la noticeÉtablissement de santé
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Dalian Medical University pays non établi dans la noticeUniversité ou école supérieure
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Second Affiliated Hospital of Chongqing Medical University Department of Respiratory Medicine and Critical Care Medicine pays non établi dans la noticeÉtablissement de santé
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Chongqing Medical University pays non établi dans la noticeUniversité ou école supérieure
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Sichuan University Department of Respiratory Medicine and Critical Care Medicine pays non établi dans la noticeUniversité ou école supérieure
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West China Hospital of Sichuan University pays non établi dans la noticeÉtablissement de santé
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Suining Central Hospital Department of Respiratory Medicine and Critical Care Medicine pays non établi dans la noticeÉtablissement de santé
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GK Health and Medical Big Data Research Center of Suining pays non établi dans la noticeStructure de recherche
Suizhou Central Hospital, Department of Respiratory Medicine and Critical Care Medicine — Fudan University et Zhongshan Hospital, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.