Isoginkgetin protects chondrocytes and inhibits osteoarthritis through NF-κB and P21 signaling pathway
Rattachement africain : cn. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
OBJECTIVE: Osteoarthritis (OA) is the most prevalent chronic articular disease in adults. The degree of cartilage degradation and matrix depletion in OA have been substantially connected with chondrocyte inflammatory response. Consequently, pharmacological anti-inflammatory agents provide OA patients a new therapeutic option. Isoginkgetin (IGK), a bioactive bioflavonoid derived from the medicinal herb Ginkgo Biloba, defends against obesity-induced heart diastolic dysfunction and harmful remodeling. Whether IGK has a regulatory effect on OA remains unknown. This study investigated whether IGK could attenuate the progression of OA both in vivo and in vitro. METHODS: Cell Counting Kit-8 (CCK8) was used to measure the vitality of chondrocytes. Mediators of inflammation, anabolism and catabolism were tested by Western blot and RT-PCR. Safranin-O staining, Hematoxylin-Eosin (H&E) staining, immunofluorescence, and Osteoarthritis Research Society International (OARSI) standards were used to assess the severity of OA and the degradation of articular cartilage. The phenotype of cartilage, NF-κB and P21 signaling pathway were measured by Western Blot. The mRNA sequencing was selected to find the differentially expressed genes and potential pathway. Pain of mice was measured by Von Frey hair mechanosensitivity. The senescence level of chondrocyte was SA-β-Gal staining. RESULTS: IGK inhibited catabolism and promoted anabolism after stimulating by IL-1β in vitro. Following destabilization of the medial meniscus (DMM) surgery, administration of IGK significantly reduced OARSI scores and attenuated AGGRECAN and COLLAGEN2 loss, overexpression of MMP3 and articular cartilage deterioration. IGK relieved pain of mice after DMM. Besides, PI3K/AKT/NF-κB, P53, Autophagy, Ferroptosis pathway and reactive oxygen species (ROS), senescence of cartilage were changed after IGK treatment. CONCLUSION: IGK protects articular cartilage and reduces the progression of OA in a mouse model and shows promise as a potential therapeutic strategy for OA.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Isoginkgetin protects chondrocytes and inhibits osteoarthritis through NF-κB and P21 signaling pathway
- Date Crossref
- 22/06/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Wuhan No.1 Hospital Department of Pharmacy pays non établi dans la noticeÉtablissement de santé
-
Wuhan No.9 Hospital pays non établi dans la noticeÉtablissement de santé
-
Huazhong University of Science and Technology pays non établi dans la noticeUniversité ou école supérieure
-
Daye People's Hospital pays non établi dans la noticeÉtablissement de santé
-
Wuhan Children's Hospital pays non établi dans la noticeÉtablissement de santé
-
Tongji Hospital pays non établi dans la noticeÉtablissement de santé
-
Union Hospital pays non établi dans la noticeÉtablissement de santé
-
Tongji Medical College Department of Clinical Nutrition pays non établi dans la noticeUniversité ou école supérieure
-
Department of Clinical Nutrition pays non établi dans la noticeÉtablissement de santé
-
the Second Affiliated of Hubei Polytechnic University Hospital Peoples of Daye City pays non établi dans la noticeUniversité ou école supérieure
Department of Pharmacy — Wuhan No.1 Hospital, Wuhan No.9 Hospital et Huazhong University of Science and Technology, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.