Phleum pratense pollen-derived di-galactosyldiacylglycerols promote pro-allergic responses in mice
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Le résumé fourni par la source
Introduction: Grass pollen triggers nearly 30% of bronchial allergic asthma cases. While most Q8 research focuses on pollen allergens, pollen lipids may also influence allergic reactions. Previous studies demonstrated that Timothy grass (TG, Phleum pratense) lipids, such as phytoprostanes, can activate immune cells, promoting pro-allergic responses. However, the role of water-insoluble pollen glycolipids in allergic airway inflammation remains unclear. Thus, this study aimed to isolate and characterize glycolipids from TG pollen and evaluate their bioactivity in allergic airway inflammation. Methods: Lipids were extracted from the water-insoluble pollen fraction, separated by silica gel, and fractionated by HPLC. GC-MS, HR ESI-MS, and NMR confirmed the presence of di-galactosyldiacylglycerol (DGDG). The biological activity of fractions containing DGDG (DGDG-3 and DGDG-4) and synthetic DGDG variants was tested in vitro in murine and human cell systems and in vivo in mice. Results: Fraction 4 induced strong proliferation of murine NKT cells and upregulated CD69 expression in human NKT cells. Synthetic DGDG variants (DGDG-1, DGDG-2, and DGDG-3) with defined acylation profiles stimulated robust NKT-cell proliferation, with DGDG-2 and DGDG-3 increasing IL-13 production, one of the key Th2 cytokines. In vivo, only these variants caused lung inflammation marked by eosinophil infiltration but did not increase airway resistance. Discussion: This study reveals for the first time the structure-dependent role of DGDG of TG pollen grains in immune cell recognition in the context of allergic inflammation. Our data may pave the way for therapies targeting lipid components in combination with protein allergens.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Phleum pratense pollen-derived di-galactosyldiacylglycerols promote pro-allergic responses in mice
- Date Crossref
- 17/06/2025
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Research Center Borstel - Leibniz Lung Center Research Group of Biofunctional Metabolites and Structures pays non établi dans la noticeStructure de recherche
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German Center for Lung Research pays non établi dans la noticeStructure de recherche
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Keio University pays non établi dans la noticeUniversité ou école supérieure
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German Center for Infection Research pays non établi dans la noticeStructure de recherche
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Faculty of Science and Technology Laboratory of Biomolecular Chemistry pays non établi dans la noticeUniversité ou école supérieure
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Thematic Translational Unit Tuberculosis pays non établi dans la noticeInstitution
Research Group of Biofunctional Metabolites and Structures — Research Center Borstel - Leibniz Lung Center, German Center for Lung Research et Keio University, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.