Glycopolymer nanomicelles: pH-responsive drug delivery, endocytosis pathway, autophagy behavior, and the effect of autophagy inhibitors
Rattachement africain : cn, us, pt. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Glycopolymer drug delivery nanosystems have attracted increasing attention in the field of sustainable biomaterials and clinical biomedicine, while few studies addressed their intracellular drug delivery properties, endocytosis pathways, intracellular trafficking, autophagy behaviors and the effect of autophagy inhibitors. Based on our previous study, in this work, a pH-responsive glycopolymer (PMAgala18-b-P(MAA24-co-MAChol6)) was synthesized and used as a drug delivery carrier, to encapsulate antitumor drug doxorubicin (DOX) into nanomicelles, with high DOX loading efficiency and pH-responsive DOX release properties. The cytotoxicity, cell proliferation inhibition, endocytosis pathway, intracellular trafficking/localization, and autophagy behavior of the blank glycopolymer micelles and/or DOX-loaded micelles were studied in a Human Glioblastoma Carcinoma (H4) and green fluorescent protein-tagged H4-GFP-LC3 cell lines. The glycopolymer micelles could be taken up into the cells through favorable caveolae-mediated and clathrin-mediated endocytic pathways, and their intracellular trafficking/localization were associated with endosome-lysosome systems. Notably, after treating with DOX-loaded glycopolymer micelles (or free DOX) to the H4-GFP-LC3 cells, exogenous substances-induced autophagosome accumulation was observed. The autophagy inhibitors: 3-methyladenine (3-MA) and hydroxychloroquine (HCQ) were used to monitor the autophagy behavior of H4-GFP-LC3 cells incubated with the micelles. Interestingly, the autophagy inhibitors could significantly enhance the antitumor performance of the free DOX and/or DOX-loaded micelles, the drug combination effect of autophagy inhibitors and DOX was further studied by Bliss independent model analysis. Taken together, this work provided a preliminary understanding of the intracellular drug delivery properties of glycopolymer micelles and demonstrated the effect of different autophagy inhibitors, which might inspire future innovation of “autophagy regulator-combined nanotherapeutics” toward efficient cancer chemotherapy.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Glycopolymer nanomicelles: pH-responsive drug delivery, endocytosis pathway, autophagy behavior, and the effect of autophagy inhibitors
- Date Crossref
- 06/06/2025
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Jinling Institute of Technology pays non établi dans la noticeUniversité ou école supérieure
-
Nebraska Medical Center pays non établi dans la noticeÉtablissement de santé
-
University of Nebraska Medical Center pays non établi dans la noticeÉtablissement de santé
-
Shanghai University Department of Polymer Materials pays non établi dans la noticeUniversité ou école supérieure
-
Centro de Química da Madeira pays non établi dans la noticeUniversité ou école supérieure
-
Universidade da Madeira CQM-Centro de Química da Madeira pays non établi dans la noticeUniversité ou école supérieure
-
School of Material Engineering pays non établi dans la noticeUniversité ou école supérieure
-
College of Pharmacy Department of Pharmaceutical Sciences pays non établi dans la noticeUniversité ou école supérieure
Jinling Institute of Technology, Nebraska Medical Center et University of Nebraska Medical Center, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.