Toward precision medicine: Inflammatory nasal epithelial transcriptomic profiles in long COVID

Le résumé fourni par la source

Rationale and objectives Little is known about the role of the nasal epithelium in long COVID. This study aimed to assess nasal epithelial transcriptomes of long COVID(LC) patients to unravel pathophysiological mechanisms for disease management. Methods Medical data and transcriptomes were obtained from participants in the ‘Precision Medicine for more Oxygen'(P4O2) COVID-19 cohort, at 3-6(n=40) and 12-18(n=15) months post-COVID. Cell type frequencies were estimated by deconvolution from a single-cell dataset. Hierarchical clustering identified transcriptomic clusters and cellular clusters from which differences in gene expression, gene set enrichment and pulmonary phenotypes were assessed. Functional validation was performed using CRISPR-Cas9 gene editing and in vitro assays in primary mutant nasal epithelium and gene expression comparisons were made to healthy controls(n=51). Results At 3-6 and 12-18 months, transcriptomes associated with inflammatory pathways(padj<0.05). Transcriptomic and cellular clusters were identified and were related to inflammation and ciliogenesis(padj<0.05). Comparison of transcriptomes of patients with and without pulmonary radiological abnormalities resulted in 613 significantly differentially expressed genes(padj<0.05). Upregulated inflammatory genes were observed in patients with abnormalities. SMURF1 expression was significantly increased in patients with compared to those without abnormalities and healthy controls. SMURF1 -/- mutant nasal epithelial cells produced significantly lower levels(p<0.05) of pro-inflammatory cytokines upon virus exposure compared to controls. Conclusion Nasal epithelium in LC exhibits persistent inflammatory states. SMURF1 upregulation potentially contributes to an exacerbated inflammatory state in nasal epithelium of patients with radiological abnormalities. This study demonstrates the importance of understanding these inflammatory profiles within a clinical context and emphasizes the need for further assessment and validation of SMURF1 's role in LC.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.