OTUD5 enhances activation of multiple cell death pathways and hyperoxia-induced lung injury by stabilizing TRAF4 and activating the p38/JNK pathway
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Le résumé fourni par la source
OBJECTIVE: This study aims to elucidate the role of OTUD5 in hyperoxia-induced lung injury and to explore its potential as a therapeutic target for mitigating oxidative stress-related lung damage. METHODS: for 24 h. Lung tissues were analyzed using histological techniques (HE, Masson's, PAS staining) and quantified for collagen deposition and airway epithelial changes. Additionally, qPCR and WB analysisWB analysis assessed OTUD5 and TRAF4 expression levels. Co-immunoprecipitation and dual-immunofluorescence assays evaluated the interaction between OTUD5 and TRAF4. Apoptosis was measured via TUNEL staining and Cleaved-Caspase-3 expression analysis, while ROS levels were assessed using MitoSOX. RESULTS: Silencing OTUD5 significantly reduced lung injury, evidenced by improved histological architecture and decreased collagen deposition. OTUD5 knockdown attenuated apoptosis markers and ROS production in BEAS-2B cells exposed to hyperoxia. Co-IP assays confirmed that OTUD5 stabilizes TRAF4, linking it to the activation of the p38/JNK signaling pathway. Furthermore, treatment with metformin and berberine chloride diminished the protective effects of OTUD5 knockdown in vivo. CONCLUSION: OTUD5 plays a critical role in hyperoxia-induced lung injury through the regulation of TRAF4 stability and apoptotic pathways. Targeting OTUD5 may provide a novel therapeutic strategy to mitigate lung damage in conditions characterized by oxidative stress.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- OTUD5 enhances activation of multiple cell death pathways and hyperoxia-induced lung injury by stabilizing TRAF4 and activating the p38/JNK pathway
- Date Crossref
- 01/10/2025
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Taizhou Second People's Hospital Department of Emergency Medicine pays non établi dans la noticeÉtablissement de santé
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Nantong University pays non établi dans la noticeUniversité ou école supérieure
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Taizhou People's Hospital pays non établi dans la noticeÉtablissement de santé
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Nanjing Medical University pays non établi dans la noticeUniversité ou école supérieure
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Jiangyan Hospital of Traditional Chinese Medicine in Taizhou City of Jiangsu Province Department of Orthopaedics pays non établi dans la noticeÉtablissement de santé
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School of Basic Medical Sciences pays non établi dans la noticeUniversité ou école supérieure
Department of Emergency Medicine — Taizhou Second People's Hospital, Nantong University et Taizhou People's Hospital, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.