Analytical quality control in targeted lipidomics: Evaluating the performance of commercial plasma as a surrogate for pooled study samples
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Le résumé fourni par la source
BACKGROUND: Pooled quality control (PQC) samples are the gold standard for data quality monitoring in metabolic phenotyping studies. Typically composed of equal parts from all study samples, PQCs can be challenging to generate in large cohorts or when sample volumes are low. As an alternative, externally sourced matrix-matched surrogate QCs (sQC) have been proposed. This study evaluates the performance of sQCs against PQCs for assessing analytical variation, data pre-processing, and downstream data analysis in a targeted lipidomics workflow. RESULTS: Plasma samples (n = 701) from the Microbiome Understanding in Maternity Study, along with PQC (n = 80) and sQC (n = 80) samples, were analyzed using a lipidomics assay targeting 1162 lipids. QC samples were injected throughout acquisition, and data pre-processing was performed using each strategy. For simplicity, a subset (n = 381) of the study samples was used to assess differences in downstream statistical analyses. Both QC approaches demonstrated high analytical repeatability. While PQC and sQC compositions differed, use of PQCs retained less than 4 % more lipid species during pre-processing. Univariate analysis identified more statistically significant lipids with PQC-based pre-processing, but multivariate model performance was similar between datasets. SIGNIFICANCE: This study provides a comprehensive comparison of QC strategies and emphasizes the importance of careful QC workflow selection. While PQCs offer advantages, sQCs serve as a suitable alternative for quality assessment and pre-processing. Their commercial availability also supports use as intra- and inter-laboratory long-term references, aiding data harmonization across studies and laboratories.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Analytical quality control in targeted lipidomics: Evaluating the performance of commercial plasma as a surrogate for pooled study samples
- Date Crossref
- 01/09/2025
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Murdoch University Health Futures Institute pays non établi dans la noticeUniversité ou école supérieure
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Fiona Stanley Hospital pays non établi dans la noticeÉtablissement de santé
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New South Wales Department of Health pays non établi dans la noticeOrganisme public
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The George Institute for Global Health pays non établi dans la noticeStructure de recherche
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Sutherland Hospital pays non établi dans la noticeÉtablissement de santé
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UNSW Sydney pays non établi dans la noticeUniversité ou école supérieure
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School of Information Technology pays non établi dans la noticeUniversité ou école supérieure
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University of Western Australia Medical School pays non établi dans la noticeUniversité ou école supérieure
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University of New South Wales Medicine and Health The George Institute for Global Health pays non établi dans la noticeUniversité ou école supérieure
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School of Clinical Medicine Discipline of Women's Health pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Medicine and Health UNSW Microbiome Research Centre pays non établi dans la noticeUniversité ou école supérieure
Health Futures Institute — Murdoch University, Fiona Stanley Hospital et New South Wales Department of Health, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.