miRNA Differential Expression Profile Analysis and Identification of Potential Key Genes in Active Tuberculosis
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Le résumé fourni par la source
Tuberculosis (TB), caused by Mycobacterium TB (MTB), remains a significant global health issue, particularly in developing nations. MicroRNAs (miRNAs) are non-coding RNAs (ncRNAs) that modulate immune responses and play a pivotal role in the pathogenesis of MTB by altering host immune defences. Insights into the regulatory functions of these miRNAs have revealed mechanisms through which MTB evades immune surveillance and establishes persistent infections, highlighting the critical role of miRNA networks in TB pathogenesis. The purpose of this study was to analyse miRNA expression in plasma from TB patients, to predict target genes, and to construct regulatory networks to elucidate the roles of miRNAs in TB pathogenesis. Plasma samples from three patients with active TB and three healthy controls were analysed using high-throughput small RNA sequencing. DEMs were identified using DESeq2, and target genes were predicted via TargetScan and miRWalk. Protein-protein interaction (PPI) networks were constructed using STRING and Cytoscape. Functional enrichment analyses were performed using Gene Ontology (GO) and KEGG databases. A total of 23 DEMs were identified, including 17 upregulated and 6 downregulated miRNAs. hsa-miR-15a-5p emerged as the most significantly upregulated miRNA. PPI network analysis highlighted CCND1, CDK6 and CCND2 as central genes, potentially regulated by miR-15a-5p. GO and KEGG analyses revealed enrichment in pathways related to cell cycle regulation, kinase activity and protein complex formation, suggesting their involvement in TB pathogenesis. This study identifies hsa-miR-15a-5p and its target genes as key components in the regulatory landscape of TB. These findings offer new insights into the molecular mechanisms of TB and propose potential biomarkers and therapeutic targets for future research.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- <scp>miRNA</scp> Differential Expression Profile Analysis and Identification of Potential Key Genes in Active Tuberculosis
- Date Crossref
- 01/05/2025
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Guilin Medical University pays non établi dans la noticeUniversité ou école supérieure
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Guangxi Academy of Special Crops pays non établi dans la noticeStructure de recherche
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Universiti Teknologi MARA pays non établi dans la noticeUniversité ou école supérieure
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The People's Hospital of Guangxi Zhuang Autonomous Region pays non établi dans la noticeÉtablissement de santé
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University of Malaya pays non établi dans la noticeUniversité ou école supérieure
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Department of Laboratory Medicine Guilin Guangxi China pays non établi dans la noticeStructure de recherche
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Nanxishan Hospital of Guangxi Zhuang Autonomous Region (The Second People's Hospital of Guangxi Zhuang Autonomous Region) Guilin Guangxi China pays non établi dans la noticeÉtablissement de santé
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Faculty of Dentistry University of Malaiya Kuala Lumpur Malaysia pays non établi dans la noticeUniversité ou école supérieure
Guilin Medical University, Guangxi Academy of Special Crops et Universiti Teknologi MARA, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.