Expression profiles analysis and roles in immunity of transient receptor potential (TRP) channel genes in Spodoptera frugiperda

Le résumé fourni par la source

BACKGROUND: Transient receptor potential (TRP) ion channels play crucial roles in mediating responses to environmental stimuli, as well as regulating homeostasis and developmental processes in insects. Several members of the TRP superfamily are potential molecular targets for insecticides or repellents, indicating their research value in pest control. This study focuses on Spodoptera frugiperda, an important invasive pest in China known for its wide host range and strong reproductive capacity. Currently, there is a lack of molecular research on the TRP channels of the invasive pest S. frugiperda. RESULTS: In this study, we identified 15 TRP family genes in S. frugiperda, which were classified into six subfamilies. The TRPP subfamily gene was not identified, whereas the TRPA subfamily contained the highest number of members in this insect. Real-time quantitative polymerase chain reaction (RT-qPCR) experiments revealed widespread expression of TRP channel genes across various developmental stages of S. frugiperda. However, TRPM and TRPML were highly expressed only in eggs. Transcripts of TRP channel genes were detected in the sensory organs of mature adults, including the mouthparts, antennae, compound eyes, legs, wings, harpagones, and ovipositors, as well as in tissues of 5th instar larvae (hemocytes, central nervous system, midgut, fat body, and Malpighian tubules). To explore the potential role of TRP channels in immunity, we detected their levels in larvae 24 h after infection with Serratia marcescens. The expression levels of TRPML, TRPL, and the Pain genes were significantly up-regulated, suggesting their important roles in immune responses to S. marcescens. CONCLUSIONS: The results of this study extend our knowledge of these critical sensory channels in S. frugiperda. This knowledge provides a basis for the future development of insecticides that target these channels, thereby promoting the safe and effective control of this key pest.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.