Abstract 4662: Tracking gene expression in the tumor microenvironment throughout breast cancer progression

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Abstract Background Genetic profiling of the tumor microenvironment involves looking at a lot of genes to figure out what roles they play in the progression of cancer and in how well treatments work. The tumor microenvironment is responsible for tumor genesis, metastasis, and resistance to standard therapy. Characterizing the tumor microenvironment through gene expression, such as ABCB1, FOXA, SFRP1, EGF, and XPB1, can provide valuable insights into the mechanisms driving cancer behavior. By understanding the interactions between these genes and their surrounding environment, researchers can develop more targeted and effective therapeutic strategies that enhance patient outcomes. This study design indicates the systematic procedure of genetic profiling, including patient enrollment and data analysis, with the objective of enhancing our comprehension of the tumor microenvironment in the specific context of Egyptian patients. Materials and Participants This study involved 60 patients, evaluating the expression levels of specific genes and identifying patterns linked to disease characteristics. RT2 Profiler PCR Arrays PASH-131Z were used to determine the gene expression of 84 genes for tumor cells and surrounding normal cells in each individual. Results: The FOXA1 gene is up-regulated significantly (p = 0.03). It is involved in the laminal classification of breast cancer and the control of transcription for genes related to estrogen hormone and estrogen receptor activity. On the other hand, genes like ABCB1, ATM, BRCA1, CCNA1, CCND2, CDK2, CDKN1A, EGF, EGFR, GATA3, IGF1R, IL6, TP53, and HPRT1 show significant down-regulation (p <0.02). These genes are involved in DNA damage and repair, cell cycle regulation, and apoptosis. Conclusion: FOXA1 and XBP1 are more important for luminal A breast cancer as a connect with hormone receptor signaling. On the other hand, SFRP and ABCB1 are more important for triple-negative breast cancer because they affect Wnt pathway activation and treatment resistance, respectively. These genes underscore the unique biological pathways present in breast cancer subtypes and demonstrate the problems and potential in their therapeutic targeting. Citation Format: Ola Sayed Ahmed, heba Morsy, Heba Ateya, Morkoss Medhat, Abdel Rahman Zekri. Tracking gene expression in the tumor microenvironment throughout breast cancer progression [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2025; Part 1 (Regular Abstracts); 2025 Apr 25-30; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2025;85(8_Suppl_1):Abstract nr 4662.

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