Small Extracellular Vesicles From Hypoxia-Neuron Maintain Blood-Brain Barrier Integrity
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Le résumé fourni par la source
BACKGROUND: Acute ischemic stroke disrupts communication between neurons and blood vessels in penumbral areas. How neurons and blood vessels cooperate to achieve blood-brain barrier repair remains unclear. Here, we reveal crosstalk between ischemic penumbral neurons and endothelial cells (ECs) mediated by circular RNA originating from oxoglutarate dehydrogenase (CircOGDH). METHODS: We analyzed clinical data from patients with acute ischemic stroke to explore the relationship between CircOGDH levels and hemorrhagic transformation events. In addition, a middle cerebral artery occlusion and reperfusion mouse model with neuronal CircOGDH suppression was used to assess endothelial permeability. ECs with increased CircOGDH expression were analyzed for changes in COL4A4 (collagen type IV alpha 4) levels, and in vitro coculture experiments were conducted to examine small extracellular vesicle-mediated CircOGDH transfer between neurons and ECs. RESULTS: Clinical data indicated that reduced CircOGDH levels were correlated with increased hemorrhagic transformation in patients with acute ischemic stroke. In the middle cerebral artery occlusion and reperfusion model, neuronal CircOGDH suppression impaired the restoration of endothelial permeability. ECs with increased CircOGDH expression exhibited higher COL4A4 levels, which helped maintain vascular stability. In vitro, hypoxic neurons transferred CircOGDH to ECs via small extracellular vesicles, leading to elevated COL4A4 expression and enhanced endothelial integrity. CONCLUSIONS: Our findings highlight the significance of CircOGDH in neuron-EC crosstalk via small extracellular vesicles in the ischemic penumbra, emphasizing the need for balanced intervention strategies in acute ischemic stroke management.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Small Extracellular Vesicles From Hypoxia-Neuron Maintain Blood-Brain Barrier Integrity
- Date Crossref
- 01/06/2025
- Éditeur
- Ovid Technologies (Wolters Kluwer Health)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Beijing Tian Tan Hospital pays non établi dans la noticeÉtablissement de santé
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National Clinical Research Center for Digestive Diseases pays non établi dans la noticeÉtablissement de santé
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First Affiliated Hospital of Jinan University Clinical Neuroscience Institute (W.C. pays non établi dans la noticeÉtablissement de santé
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First People's Hospital of Foshan Department of Neurology pays non établi dans la noticeÉtablissement de santé
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The First People's Hospital of Shunde pays non établi dans la noticeÉtablissement de santé
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Sun Yat-sen University Department of Neurology pays non établi dans la noticeUniversité ou école supérieure
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Sun Yat-sen Memorial Hospital pays non établi dans la noticeÉtablissement de santé
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China National Clinical Research Center for Neurological Diseases pays non établi dans la noticeStructure de recherche
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Capital Medical University Department of Neurology pays non établi dans la noticeUniversité ou école supérieure
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Key Laboratory of Guangzhou Basic and Translational Research of Pan-Vascular Diseases (W.C. pays non établi dans la noticeStructure de recherche
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The Affiliated Shunde Hospital of Jinan University Department of Neurology pays non établi dans la noticeUniversité ou école supérieure
Beijing Tian Tan Hospital, National Clinical Research Center for Digestive Diseases et Clinical Neuroscience Institute (W.C. — First Affiliated Hospital of Jinan University, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.