Overexpressing OsCCT23 Delays Heading Date and Increases Grain Yield by Activating Ghd7 in Rice
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Le résumé fourni par la source
Heading date and panicle architecture are pivotal traits that significantly influence rice yield. Here, we identified a gene OsCCT23 encoding a CCT domain-containing protein that delays heading by over 40 days and increases grain yield by 60-104% through overexpressing. Two types transcripts OsCCT23L and OsCCT23S were isolated by 5'RACE, and transgenic events demonstrated that the effect of the predominant transcript OsCCT23S, encoding an 81-aa protein without the B-box domain, is comparable to OsCCT23. OsCCT23 is predominantly expressed in leaves and follows a diurnal expression pattern with a peak at dawn. Overexpressing OsCCT23 upregulated the floral repressor Ghd7 and downregulated the floral inducer RID1, consequently led to the downregulation of Ehd1, Hd3a and RFT1. Additionally, it regulates the expression of certain circadian clock-related genes, including OsGI and OsTOC1. RNA in situ hybridisation analysis confirmed that OsCCT23 activates the expression of Ghd7 in the panicle branch meristem. OsCCT23 suppresses the expression of four OsCKX genes including Gn1a, which associate with cytokinin accumulation in panicles. Natural variation in OsCCT23 promoter identified by eGWAS associates its mRNA abundance and rice heading date. Consequently, OsCCT23 substantially delays heading and significantly increases grain yield, making it highly valuable for rice breeding.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Overexpressing <i>OsCCT23</i> Delays Heading Date and Increases Grain Yield by Activating <i>Ghd7</i> in Rice
- Date Crossref
- 30/03/2025
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Chinese Academy of Agricultural Sciences pays non établi dans la noticeOrganisme public
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Biotechnology Research Institute pays non établi dans la noticeStructure de recherche
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Huazhong Agricultural University National Key Laboratory of Crop Genetic Improvement pays non établi dans la noticeUniversité ou école supérieure
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Jiangsu Provincial Academy of Forestry Science and Management pays non établi dans la noticeInstitution
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Yangtze University MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province) pays non établi dans la noticeUniversité ou école supérieure
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Jiangsu Academy of Forestry Nanjing China pays non établi dans la noticeInstitution
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Agricultural College Engineering Research Center of Ecology and Agricultural Use of Wetland Ministry of Education pays non établi dans la noticeUniversité ou école supérieure
Chinese Academy of Agricultural Sciences, Biotechnology Research Institute et National Key Laboratory of Crop Genetic Improvement — Huazhong Agricultural University, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.