Dietary whey protein protects against Crohn’s disease by orchestrating cross-kingdom interaction between the gut phageome and bacteriome
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Le résumé fourni par la source
Background The gut microbiome and diet are important factors in the pathogenesis and management of Crohn’s disease (CD). However, the role of the gut phageome under dietary influences is unknown. Objective We aim to explore the effect of diet on the gut phageome-bacteriome interaction linking to CD protection. Design We recruited CD patients and healthy subjects (n=140) and conducted a multiomics investigation, including paired ileal mucosa phageome and bacteriome profiling, dietary survey and phenome interrogation. We screened for the effect of diet on the gut phageome and bacteriome, as well as its epidemiological association with CD risks. The underlying mechanisms were explored in target phage-bacteria monocultures and cocultures in vitro and in two mouse models in vivo. Results On dietary screening in humans, whey protein (WP) consumption was found to profoundly impact the gut phageome and bacteriome (more pronounced on the phageome) and was associated with a lower CD risk. Indeed, the WP reshaped gut phageome can causally attenuate intestinal inflammation, as shown by faecal phageome versus bacteriome transplantation from WP-consuming versus WP-non-consuming mice to recipient mice. Mechanistically, WP induced phage (a newly isolated phage AkkZT003P herein) lysis of the mucin-foraging bacterium Akkermansia muciniphila , which unleashed the symbiotic bacterium Streptococcus thermophilus to counteract intestinal inflammation. Conclusion Our study charted the importance of cross-kingdom interaction between gut phage and bacteria in mediating the dietary effect on CD protection. Importantly, we uncovered a beneficial dietary WP, a keystone phage AkkZT003P, and a probiotic S. thermophilus that can be used in CD management in the future.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Dietary whey protein protects against Crohn’s disease by orchestrating cross-kingdom interaction between the gut phageome and bacteriome
- Date Crossref
- 23/03/2025
- Éditeur
- BMJ
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Sun Yat-sen University The Sixth Affiliated Hospital pays non établi dans la noticeUniversité ou école supérieure
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Sixth Affiliated Hospital of Sun Yat-sen University pays non établi dans la noticeÉtablissement de santé
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Central South University Department of Gastroenterology pays non établi dans la noticeUniversité ou école supérieure
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Third Xiangya Hospital pays non établi dans la noticeÉtablissement de santé
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Jinan University pays non établi dans la noticeUniversité ou école supérieure
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Shandong University pays non établi dans la noticeUniversité ou école supérieure
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Shandong Provincial QianFoShan Hospital Department of Gastroenterology pays non établi dans la noticeÉtablissement de santé
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Shandong First Medical University pays non établi dans la noticeUniversité ou école supérieure
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Second Affiliated Hospital of Zhejiang University pays non établi dans la noticeÉtablissement de santé
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Sichuan University Department of Gastroenterology pays non établi dans la noticeUniversité ou école supérieure
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West China Hospital of Sichuan University pays non établi dans la noticeÉtablissement de santé
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Institute of Bioorganic Chemistry pays non établi dans la noticeStructure de recherche
The Sixth Affiliated Hospital — Sun Yat-sen University, Sixth Affiliated Hospital of Sun Yat-sen University et Department of Gastroenterology — Central South University, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.