Dissecting the Sensitization Profiles in Parvalbumins From 12 Freshwater Fish Species to Improve Diagnosis of Fish Allergy
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Le résumé fourni par la source
BACKGROUND AND OBJECTIVES: Background: Many fish-allergic patients only react to certain fish species and may tolerate others, mostly because of IgE-mediated recognition of specific epitopes on the major allergen parvalbumin. However, the considerable number of fish species consumed makes it challenging to identify which species are allergenic and which are tolerated by individual patients. Objective: In order to improve the diagnosis of fish allergy, we investigated IgE-mediated reactivity to parvalbumins from 12 freshwater fish species that are largely underrepresented in diagnostic tests. METHODS: Parvalbumins were purified from 12 freshwater fish species belonging to 8 families, and their isoform composition was analyzed using mass spectrometry. IgE specific for each parvalbumin was quantified in serum samples from 66 fish-allergic individuals, and basophil activation tests were performed for 5 patients. Crosswise inhibition assays were carried out for all parvalbumins for 7 patients to investigate cross-reactivity between the parvalbumins from the different species. RESULTS: IgE binding and cross-linking potency of the parvalbumins differed, with the strongest reactivities observed for 4 parvalbumins from the salmonid family (results positive for 89%-95% of patients) and the weakest for parvalbumins from Wels catfish, European eel, and tench (results negative for ≥50% of patients). Ninety percent of the patients with negative results for Wels catfish parvalbumin also had negative results for additional parvalbumins from multiple species. Inhibition assays revealed variable recognition of epitopes by several patients, with the primary sensitizers most frequently being parvalbumins from salmonids and percids. CONCLUSIONS: Including freshwater salmonids in the diagnostic work-up for fish allergy may help to identify most fish-allergic patients. IgE to Wels catfish could help distinguish between polysensitized and oligosensitized patients.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Dissecting the Sensitization Profiles in Parvalbumins From 12 Freshwater Fish Species to Improve Diagnosis of Fish Allergy
- Date Crossref
- 15/12/2025
- Éditeur
- Esmon Publicidad, SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universitätsklinikum St. Pölten pays non établi dans la noticeÉtablissement de santé
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Karl Landsteiner University of Health Sciences pays non établi dans la noticeUniversité ou école supérieure
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Medical University of Vienna Institute of Pathophysiology and Allergy Research pays non établi dans la noticeUniversité ou école supérieure
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University of Belgrade pays non établi dans la noticeUniversité ou école supérieure
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Yonsei University Department of Internal Medicine pays non établi dans la noticeUniversité ou école supérieure
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University Hospital St. Pölten Department of Dermatology pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Medicine Institute of Medical Chemistry pays non établi dans la noticeUniversité ou école supérieure
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MacroArray Diagnostics pays non établi dans la noticeInstitution
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Faculty of Chemistry Department of Biochemistry pays non établi dans la noticeUniversité ou école supérieure
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Floridsdorf Allergy Center pays non établi dans la noticeInstitution
Universitätsklinikum St. Pölten, Karl Landsteiner University of Health Sciences et Institute of Pathophysiology and Allergy Research — Medical University of Vienna, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.