Solid evidence and liquid gold: trade-offs of processing settled solids, whole influent, or centrifuged influent for co-detecting viral, bacterial, and eukaryotic pathogens in wastewater
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Le résumé fourni par la source
Abstract Effective methods for simultaneously measuring viral, bacterial, protozoan, and fungal pathogens in wastewater are needed. Here, we investigate how sample type and nucleic acid extraction protocols affect broad-range pathogen detection. We compared methods for analyzing wastewater solids and whole influent by dPCR detection of spiked and endogenous targets including DNA and RNA viruses (mpox, norovirus, SARS-CoV-2), bacteria ( Clostridium difficile, Campylobacter jejuni ), protozoa ( Cryptosporidium spp .), fungi ( Candida auris ), and antibiotic resistance genes. Using selected methods, we then analyzed date-matched 1) solid, 2) centrifuged influent, and 3) whole influent samples collected from eleven facilities at three time points. We demonstrate that one workflow can be used to simultaneously detect all targets and that all sample types yielded similar detection levels and nucleic acid concentrations. Comparing normalization of targets by concentration of PMMoV, carjivirus, and 16S, we show that using different controls together can complicate interpretation of concentrations across targets. Centrifuged influent produced comparable or higher target concentrations overall, suggesting that centrifuged influent is a viable option when settled solids are not available and can circumvent the limitation of varying residence times for primary settled solids. Abstract Figure Synopsis We report successful simultaneous detection of viral, bacterial, and eukaryotic pathogens in both solid and liquid wastewater samples and explore multiple ways to compare sample concentrations measured in different matrices.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Solid evidence and liquid gold: trade-offs of processing settled solids, whole influent, or centrifuged influent for co-detecting viral, bacterial, and eukaryotic pathogens in wastewater
- Date Crossref
- 05/03/2025
- Éditeur
- openRxiv
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of California Department of Civil and Environmental Engineering pays non établi dans la noticeUniversité ou école supérieure
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University of Washington Department of Environmental and Occupational Health Sciences pays non établi dans la noticeUniversité ou école supérieure
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Lawrence Livermore National Laboratory Physical and Life Sciences Directorate pays non établi dans la noticeStructure de recherche
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Chan Zuckerberg Initiative (United States) pays non établi dans la noticeEntreprise
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Chan Zuckerberg Biohub San Francisco pays non établi dans la noticeInstitution
Department of Civil and Environmental Engineering — University of California, Department of Environmental and Occupational Health Sciences — University of Washington et Physical and Life Sciences Directorate — Lawrence Livermore National Laboratory, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.